Recombinant antigens for immunodiagnosis of cystic echinococcosis

被引：35

作者：

Li J. ^{[1
]}

Zhang W.-B. ^{[1
]}

McManus D.P. ^{[1
]}

机构：

[1] Molecular Parasitology Laboratory, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research, Brisbane, QLD 4029

来源：

Biological Procedures Online | 2004年 / 6卷 / 1期

关键词：

Blotting; Western; Echinococcus; Recombinant proteins;

D O I：

10.1251/bpo74

中图分类号：

学科分类号：

摘要：

Three cDNAs, termed EpC1, TPxEg and EgG5, were isolated by immunoscreening from an Echinococcus granulosus cDNA library. The recombinant phages exhibited strong reactivity with sera from humans with confirmed cystic echinococcosis (CE) and with sera from mice infected with E. granulosus oncospheres. The cDNAs were subcloned into a pET vector, expressed as fusion proteins tagged with GST and affinity purified against the GST tag. Of the three recombinant proteins, EpC1 achieved the highest performance for serodiagnosis of CE in Western blot analysis using a panel of clinically defined human sera to initially address the sensitivity and specificity of the molecules. The protein yielded an overall sensitivity of 92.2% and specificity of 95.6%, levels unprecedented taking into account the large panel of 896 human sera that were tested. The strategy used may also prove suitable for improved immunodiagnosis of other parasitic infections. © 2004 Biological Procedures Online.

引用

页码：67 / 77

页数：10

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