Effect of moxibustion on expression of HSP70 and apoptosisrelated factors in rats with acute gastric mucosal damage

被引：3

作者：

Yi S.-X. ^{[1
]}

Yu J. ^{[1
]}

Chang X.-R. ^{[1
]}

Peng Y. ^{[1
]}

Wu H.-G. ^{[2
]}

Yan J. ^{[1
]}

Yang R.-D. ^{[1
]}

机构：

[1] College of Acupuncture and Massage, Hunan University of Traditional Chinese Medicine

[2] Shanghai Research Institute of Acupuncture and Meridian

来源：

Journal of Acupuncture and Tuina Science | 2010年 / 8卷 / 1期

基金：

中国国家自然科学基金;

关键词：

Apoptosis; Caspases; Gastric Mucosa; HSP70 Heat-Shock Proteins; Mitochondria; Moxibustion; Rats;

D O I：

10.1007/s11726-010-0005-z

中图分类号：

学科分类号：

摘要：

Objective: To observe the effect of preventively used moxibustion on the expression of apoptosis-related factors in rats with acute gastric mucosal damage (GMD), and analyze the relationship between those factors and heat shock protein 70 (HSP70), for discovering the mechanism of moxibustion for protecting gastric mucosa from the aspect of mitochondria signal transduction of apoptosis. Methods: Thirty-two Wistar rats were randomly divided into binding, model, acupoint-moxibustion and non-acupoint-moxibustion groups, 8 in each group. Moxibustion was applied preventively to acupoint-moxibustion and non-acupointmoxibustion groups respectively for 8 d. Rats in binding and model groups were served with restraint without moxibustion. The GMD models were induced in groups except for A group, and the expression of cytochrome c (Cyt-c) in gastric mucosa was measured by Western-blot analysis, the expression of HSP70, cell apoptosis index (AI), apoptotic protease activating factor 1 (Apaf-1), Caspase-9, Caspase-3, Bcl-2 and Bax in gastric mucosal cells were studied by immunohistochemical detection. Results: Compared with binding group, the expressions of HSP70, AI, Cyt-c, Apaf-1, Caspase-9, Caspase-3, Bcl-2 and Bax of gastric mucosa in model group were significantly higher (P<0.05 or P<0.01). Compared with model group, the expressions of HSP70 and Bcl-2 were significantly higher (P<0.01), while AI, Cyt-c, Apaf-1, Caspase-9, Caspase-3, Bax were significantly lower (P<0.01) in acupoint-moxibustion group. Compared with the acupoint-moxibustion group, the expressions of HSP70 and Bcl-2 were significantly lower (P<0.01), while AI, Cyt-c, Apaf-1, Caspase-9, Caspase-3, Bax were significantly higher (P<0.05 or P<0.01) in non-acupoint-moxibustion group. Conclusion: Moxibustion inhibits the apoptosis of rats' gastric mucosal cells and protect them from damage, through up-regulating the expression of HSP70, and modulating the expressions of Cyt-c, Apaf-1, Caspase-9, Caspase-3, Bcl-2 and Bax which were related to the mitochondria signal transduction of apoptosis. © Shanghai Research Institute of Acupuncture and Meridian and Springer Berlin Heidelberg 2010.

引用

页码：5 / 11

页数：6

共 35 条

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