DNA isolation from fresh and dry plant samples with highly acidic tissue extracts

被引：26

作者：

Dnyaneshwar Warude

Preeti Chavan

Kalpana Joshi

Bhushan Patwardhan

机构：

[1] Interdisc. School of Health Sciences, University of Pune, Ganeshkhind

来源：

Plant Molecular Biology Reporter | 2003年 / 21卷 / 4期

关键词：

Ayurveda; DNA isolation; Emblica officinalis; Low pH; PCR; Terminalia belerica; Terminalia chebula; Triphala churna;

D O I：

10.1007/BF02772600

中图分类号：

学科分类号：

摘要：

Current DNA isolation methods have limitations in their ability to obtain quality and/or quantity DNA from plants, such as Emblica officinalis, Terminalia belerica, and Terminalia chebula, which have low pH and high amounts of secondary metabolites in tissue extracts. Our modified DNA isolation method yields good-quality, high-molecularweight DNA that is free of contaminants and colored pigments and is suitable for PCR amplification. This method is also useful for isolating DNA from dry powders.

引用

页码：467a / 467f

共 15 条

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