Reduction of post injury neointima formation due to 17β-estradiol and phytoestrogen treatment is not influenced by the pure synthetic estrogen receptor antagonist ICI 182,780 in vitro

被引：7

作者：

Finking G. ^{[1
]}

Lenz C. ^{[2
]}

Schochat T. ^{[1
]}

Hanke H. ^{[2
]}

机构：

[1] Inst. Occup. Social/Environ. Med., University of Ulm, Ulm

[2] Department of Cardiology, University of Ulm, Ulm

来源：

BMC Cardiovascular Disorders | / 2卷 / 1期

关键词：

17β-estradiol; ICI 182,780 estrogen receptor; Phytoestrogens; Vascular injury;

D O I：

10.1186/1471-2261-2-13

中图分类号：

学科分类号：

摘要：

Background: Animal and organ culture experiments have shown beneficial inhibitory estrogen effects on post injury neointima development. The purpose of this study was to investigate whether such estrogen effects are influenced by the estrogen receptor antagonist ICI 182,780. Different concentrations of 17β-estradiol and the phytoestrogens genistein and daidzein were tested. Methods: Female New Zealand White rabbits were benumbed. In situ vascular injury of the thoracic and abdominal aorta was performed by a 3F Fogarty catheter. Segments of 5 mm were randomised and held in culture for 21 days. Three test series were performed: 1) control group - 20 μ M ICI - 30 μM ICI - 40 μM ICI. 2) control group - 20 μM ICI - 40 μM 17β-estradiol - 40 μM 17β-estradiol + 20 μM ICI. 3 control group - 20 μM ICI - 40 μM daidzein - 40 μM daidzein + 20 μM ICI - 20 μM genistein - 20 μM genistein + 20 μM ICI. After 21 days the neointima-media-ratio was evaluated. Results: 1) Treatment with ICI 182,780 did not reduce neointima formation significantly (p = 0.05). 2) 40 μM 17β-estradiol alone (p < 0.0001) and in combination with 20 μM ICI (p < 0.0001) reduced neointima formation significantly. 3) 20 μM genistein alone (p = 0.0083) and combined with 20 μM ICI (p = 0.0053) reduced neointima formation significantly. 40 μM daidzein did not have a significant (p = 0.0637) effect. Conclusions: The estrogen receptor antagonist ICI 182,780 did not modulate the inhibitory estrogen effects on post injury neointima formation. These results do not support the idea that such effects are mediated by vascular estrogen receptors. © 2002 Finking et al; licensee BioMed Central Ltd.

引用

页数：7

共 50 条

[1]

Mendelsohn M.E., Karas R.H., The protective effects of estrogen on the cardiovascular system, N. Engl. J. Med., 340, pp. 1801-1811, (1999)

[2]

Finking G., Gohar M.H., Lenz C., Hanke H., Die Wirkungen von Ostrogen im kardiovaskularen System, Z. Kardiol., 89, pp. 442-453, (2000)

[3]

Finking G., Gohar M.H., Lenz C., Hanke H., Cardiovascular oestrogen effects in women, J. Obstet. Gynaecol., 21, pp. 340-349, (2001)

[4]

Sullivan J.M., Vander-Zwaag R., Hughes J.P., Maddock V., Kroetz F.W., Ramanathan K.B., Mirvis D.M., Estrogen replacement and coronary artery disease. Effect on survival in postmenopausal women, Arch. Intern. Med., 150, pp. 2557-2562, (1990)

[5]

Grodstein F., Stampfer M.J., Colditz G.A., Willett W.C., Manson J.E., Joffe M., Rosner B., Fuchs C., Hankinson S.E., Hunter D.J., Hennekens C.H., Speizer F.E., Postmenopausal hormone therapy and mortality, N. Engl. J. Med., 336, pp. 1769-1775, (1997)

[6]

Hulley S., Grady D., Bush T., Furberg C., Herrington D., Riggs B., Vittinghoff E., Randomized trial of estrogen plus progestin for secondary prevention of coronary heart disease in postmenopausal women, Jama, 280, pp. 605-613, (1998)

[7]

O'Brien J.E., Peterson E.D., Keeler G.P., Berdan L.G., Ohman E.M., Faxon D.P., Jacobs A.K., Topol E.J., Califf R.M., Relation between estrogen replacement therapy and restenosis after percutaneous coronary interventions, J. Am. Coll. Cardiol., 28, pp. 1111-1118, (1996)

[8]

O'Keefe J.H., Kim S.C., Hall R.R., Cochran V.C., Lawhorn S.L., McCallister B.D., Estrogen replacement therapy after coronary angioplasty in women, J. Am. Coll. Cardiol., 29, pp. 1-5, (1997)

[9]

Effects of estrogen or estrogen/progestin regimens on heart disease risk factors in postmenopausal women, Jama, 273, pp. 199-208

[10]

Sack M.N., Rader D.J., Cannon R.O., Oestrogen and inhibition of oxidation of low-density lipoproteins in postmenopausal women, Lancet, 343, pp. 269-270, (1994)

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