Induced ovulation and egg deposition in the direct developing anuran Eleutherodactylus coqui

被引：22

作者：

Michael S. ^{[1
]}

Buckley C. ^{[2
]}

Toro E. ^{[3
]}

Estrada A.R. ^{[4
]}

Vincent S. ^{[2
]}

机构：

[1] Department of Tropical Medicine, Tulane University, New Orleans, LA

[2] Dept. of Ecol./Evolutionary Biology, Tulane University, New Orleans, LA

[3] Departamento de Ciencias Biologicas, Universidad de los Andes, Bogotá

[4] Dept. of Science Technology/Health, Universidad Metropolitana, San Juan

来源：

Reproductive Biology and Endocrinology | / 2卷 / 1期

关键词：

Xenopus Laevis; Amphibian Species; Reproductive Hormone; Anuran Species; External Fertilization;

D O I：

10.1186/1477-7827-2-6

中图分类号：

学科分类号：

摘要：

This study investigates ovulation and egg deposition behaviors in the anuran Eleutherodactylus coqui from Puerto Rico in response to stimulation with gonadotropin and gonadotropin releasing hormones. Five hormones were tested by injection over a range of doses, including mammalian LHRH, avian LHRH, fish LHRH, D-Ala6, des-Gly10 ethylamide LHRH and hCG. We report a low level of ovulation and egg deposition in response to all hormones, with the most complete and consistent results from the non-natural D-Ala6, des-Gly10 ethylamide LHRH derivative. To confirm the viability of eggs produced in this manner we performed in vitro fertilization experiments that resulted in the development of normal frogs. Reproductive behaviors in E. coqui are apparently not controlled by a mammalian form of LHRH as reported in other common laboratory anuran species. D-Ala6, des-Gly10 ethylamide LHRH induces ovulation and deposition of mature and fertilizable eggs in E. coqui. © 2004 Michael et al; license BioMed Central Ltd.

引用

页数：5

共 21 条

[1]

Lynch J.D., Replacement names for three homonyms in the genus Eleutherodactylus (Anura: Leptodactylidae), J. Herpetol., 30, pp. 278-280, (1996)

[2]

Wake M.H., The reproductive biology of Eleutherodactylus jasperi (Amphibia, anura, Leptodactylidae), with comments on the evolution of live-bearing systems, J. Herpetol., 12, pp. 121-133, (1978)

[3]

Townsend D.S., Patterns of parental care in frogs of the genus Eleutherodactylus, Contributions to West Indian Herpetology: A Tribute to Albert Schwartz, pp. 229-239, (1996)

[4]

Stewart M.M., Pough F.H., Population density of tropical forest frogs: Relation to retreat sites, Science, 221, pp. 570-572, (1983)

[5]

Narins P.M., Capranica R.R., Sexual differences in the auditory system of the tree frog Eleutherodactylus coqui, Science, 192, pp. 378-380, (1976)

[6]

Stewart M.M., Rand A.S., Vocalizations and the defense of retreat sites by male and female frogs, Eleutherodactylus coqui, Copeia, 1991, pp. 1013-1024, (1991)

[7]

Michael S.F., Courtship calls of three species of Eleutherodactylus from Puerto Rico (Anura: Leptodactylidae), Herpetologica, 52, pp. 116-120, (1996)

[8]

Elinson R.P., Direct development: An alternative way to make a frog, Genesis: The Journal of Genetics & Development, 29, pp. 91-95, (2001)

[9]

Hanken J., Carl T.F., Richardson M.K., Olsson L., Schlosser G., Osabutey C.K., Klymkowsky M.W., Limb development in a "nonmodel" vertebrate, the direct-developing frog Eleutherodactylus coqui, J. Exp. Zool., 291, pp. 375-388, (2001)

[10]

Creaser C.W., Gorbman A., Species specificity of the gonadotropic factors in vertebrates, Quart Rev. Biol., 14, pp. 311-331, (1939)

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