The role of intracellular cAMP in mediating the synchronizing action of noradrenaline on the evoked release of quanta of mediator in the frog synapse

被引：2

作者：

Bukharaeva É.A. ^{[1
,2
]}

Samigullin D.V. ^{[2
]}

Nikol'skii E.E. ^{[1
,2
]}

Vyskochil F. ^{[3
]}

机构：

[1] Kazan' State Medical University, 420012 Kazan

[2] Kazan' Institute of Biochemistry and Biophysics, 420503 Kazan

[3] Charles University, Prague

来源：

Neuroscience and Behavioral Physiology | 2001年 / 31卷 / 5期

基金：

俄罗斯基础研究基金会;

关键词：

Adenylate cyclase; Cyclic AMP; Modulation of evoked secretion; Neuromuscular synapse; Noradrenaline; Synchronization of quantum mediator secretion;

D O I：

10.1023/A:1010414612669

中图分类号：

学科分类号：

摘要：

Experiments on frog neuromuscular junction preparations with extracellular recording of action currents in nerve endings and single-quantum currents from endplates were used to assess the time course of evoked quantum mediator secretion by analyzing histograms showing the distribution of true synaptic delays. Studies using the cyclic AMP analog dibutyryl-cAMP (db-cAMP), the adenylate cyclase activator forskolin, and the nucleotide-dependent phosphodiesterase inhibitor isobutylmethylxanthine, showed that these agents, like noradrenaline, altered the kinetics of secretion of quanta, leading to synchronization of the release of mediator. After preliminary treatment of the neuromuscular preparation with db-cAMP, forskolin, or isobutylmethylxanthine, noradrenaline did not induce the synchronization of mediator release in quanta. It was concluded that the action of noradrenaline on the time course of secretion is mediated by activation of presynaptic β receptors, increased adenylate cyclase activity, and increases in intracellular cAMP levels.

引用

页码：473 / 480

页数：7

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