Cell proliferation in the adult rat rostral migratory stream following exposure to gamma irradiation

被引：21

作者：

Soňa Bálentová ^{[1
]}

Enikö Račeková ^{[3
]}

Marcela Martončíková ^{[2
]}

Eva Mišúrová ^{[2
]}

机构：

[1] Institute of Biology and Ecology, Faculty of Science, P.J. Šafárik University, 040 01 Košice

[2] Institute of Neurobiology, Centre of Excellence, Slovak Academy of Sciences, 040 01 Košice

来源：

Cellular and Molecular Neurobiology | 2006年 / 26卷 / 7期

关键词：

Cell proliferation; Irradiation; Rat brain; Rostral migratory stream;

D O I：

10.1007/s10571-006-9067-3

中图分类号：

学科分类号：

摘要：

One of the few areas of the adult CNS, that are known to be competent for neuronal proliferation, is the subventricular zone (SVZ) lining the brain lateral ventricles. Cells proliferating in the SVZ migrate along a defined pathway, the rostral migratory stream (RMS), where their proliferation continues until reaching the olfactory bulb. 1. In relation to the fact that brain is, in general, regarded as a radioresistant organ composed from non dividing cells, the aim of the present study was to investigate effect of ionizing radiation on proliferating cell numbers in the RMS of adult rats. 2. Male Wistar rats were investigated 25 and 80 days after whole body gamma irradiation with the dose of 3 Gy. Dividing cells were labeled by bromodeoxyuridine (BrdU). BrdU-positive cells were counted by Disector program. The mean number of BrdU+ cells in the whole RMS and in its individual parts (vertical arm, elbow, and horizontal arm) was evaluated. 3. Temporary increase in proliferating cell number (by 30%) was seen in the whole RMS at the 25th day after irradiation. 4. The most expressive increase occurred in the vertical arm (by 60%) and elbow (about 37%). The values reduced till the 80th day after exposure. Our results show that ionizing irradiation significantly influences the extent of cell proliferation and migration in the adult rat RMS. © 2006 Springer Science+Business Media, Inc.

引用

页码：1131 / 1139

页数：8

共 25 条

[1]

Alvarez-Buylla A., Temple S., Stem cells in the developing and adult nervous system, J. Neurobiol., 36, pp. 105-110, (1998)

[2]

Amano T., Inamura T., Wu C.M., Kura S., Nakamizo A., Inoha S., Miyazono M., Ikezaki K., Effects of single low dose irradiation on subventricular zone cells in juvenile brain, Neurol. Res., 24, pp. 809-816, (2002)

[3]

Cayre M., Malaterre J., Scotto-Lomassese S., Strambi C., Strambi A., The common properties of neurogenesis in the adult brain: From invertebrates to vertebrates, Comp. Biochem. Physiol. B. Biochem. Mol. Biol., 132, pp. 1-15, (2002)

[4]

Chua K., Kima M., Parka K.I., Jeongc S.W., Parka H.K., Junga K.H., Leea S.T., Kanga L., Leea K., Parka D.K., Kimd S.U., Roh J.K., Human neural stem cells improve sensorimotor deficits in the adult rat brain with experimental focal ischemia, Brain Res., 1016, pp. 145-153, (2004)

[5]

Dasika G.K., Lin S.-C.J., Zhao S., Sung P., Tomkinson A., Kee E.Y.-H., DNA damage-induced cell cycle checkpoints and DNA strand break repair in development and tumorigenesis, Oncogene, 18, pp. 7883-7899, (1999)

[6]

Doetsch F., A niche for adult neural stem cells, Curr. Opin. Genet. Dev., 13, pp. 543-550, (2003)

[7]

Jankovski A., Garcia C., Soriano E., Sotelo C., Proliferation, migration and differentiation of neuronal progenitor cells in the adult subventricular zone surgically separated from its olfactory bulb, Eur. J. Neurosci., 10, pp. 3853-3868, (1998)

[8]

Jankovski A., Sotelo C., Subventricular zone-olfactory bulb migratory pathway in the adult mouse: Cellular composition and specificity as determined by heterochronic and heterotopic transplantation, J. Comp. Neurol., 371, pp. 376-396, (1996)

[9]

Kee N., Sivalingam S., Boonstra R., Wojtowicz J.M., The utility of Ki-67 and BrdU as proliferative markers of adult neurogenesis, J. Neurosci., 115, pp. 97-105, (2002)

[10]

Kempermann G., Why new neurons? Possible functions for adult hippocampal neurogenesis, J. Neurosci., 22, pp. 635-638, (2002)

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