Analysis of ENOD40 expression in alb1, a symbiotic mutant of Lotus japonicus that forms empty nodules with incompletely developed nodule vascular bundles

被引：29

作者：

Imaizumi-Anraku H. ^{[1
,2
,3
]}

Kouchi H. ^{[2
]}

Syono K. ^{[4
]}

Akao S. ^{[2
]}

Kawaguchi M. ^{[3
]}

机构：

[1] Laboratory of Disease Physiology, Department of Plant Physiology, National Institute of Agrobiological Resources, Tsukuba, 305-8602

[2] Laboratory of Nitrogen Fixation, Department of Plant Physiology, National Institute of Agrobiological Resources, Tsukuba, 305-8602

[3] Department of Life Science, Tokyo University, Meguro-ku, Tokyo, 153-8902

[4] Department of Science, Japan Women's University, Bunkyo-ku, Tokyo, 112-8681

来源：

Molecular Genetics and Genomics | 2000年 / 264卷 / 4期

基金：

日本学术振兴会;

关键词：

alb1; ENOD40; Lotus japonicus; Nodule organogenesis; Nodule vascular bundle; Symbiosis-defective mutant;

D O I：

10.1007/s004380000330

中图分类号：

学科分类号：

摘要：

The alb1 mutant of Lotus japonicus (Ljsym74) forms empty nodules in which most of the bacteria remain in abnormally enlarged infection threads and fail to enter the host plant cells. The alb1 mutant was also found to be defective in differentiation of ramified nodule vascular bundles; only a single vascular bundle differentiates at the proximal end of the alb1 nodules and it fails to differentiate further. Histochemical analysis using fluorescein-conjugated wheat-germ agglutinin (F-WGA) indicated that the mutation in the ALB1 gene specifically affects the differentiation of vascular bundles in nodules. Analysis of nodulin gene expression revealed that the expression of an early nodulin gene, ENOD40, was very low in alb1 nodules. At early developmental stages of alb1 nodules, the pattern of ENOD40 transcription was essentially the same as that in wild-type nodules; transcripts were localized in dividing cortical cells and in the pericycle of the root stele opposite nodule primordia, as in wild-type nodules. However, mature alb1 nodules exhibited very weak or no expression of ENOD40 in the peripheral cells of the undeveloped nodule vascular bundle. The ENOD40 expression pattern in alb1 nodules is distinct from that in another ineffective mutant, fen1 (Ljsym76), in which ENOD40 expression persists prior to premature senescence. These findings lead us to speculate that ENOD40 may play a role in the differentiation of nodule vascular bundles.

引用

页码：402 / 410

页数：8

共 25 条

[1]

Ardourel M., Demont N., Debelle F.D., Maillet F., Debilly F., Prome J.C., Denarie J., Truchet G., Rhizobium meliloti lipooligosaccharide nodulation factors: Different structural requirements for bacterial entry into target root hair cells and induction of plant symbiotic developmental responses, Plant Cell, 6, pp. 1357-1374, (1994)

[2]

Charon C., Johansson C., Kondorosi E., Kondorosi A., Crespi M., enod40 induces dedifferentiation and division of root cortical cells in legumes, Proc Natl Acad Sci USA, 94, pp. 8901-8906, (1997)

[3]

Charon C., Sousa C., Crespi M., Kondorosi A., Alteration of enod40 expression modifies Medicago truncatula root nodule development induced by Sinorhizobium meliloti, Plant Cell, 11, pp. 1953-1966, (1999)

[4]

Crespi M., Jurkevitch E., Poiret M., D'Aubenton-Carafa Y., Petrovics G., Kondorosi E., Kondorosi A., enod40, a gene expressed during nodule organogenesis, codes for a non-translatable RNA involved in plant growth, EMBO J, 13, pp. 5099-5112, (1994)

[5]

Downie J.A., The Rhizobiaceae - molecular biology of model plant-associated bacteria, pp. 387-402, (1998)

[6]

Flemetakis M., Kavroulakis P., Katinakis P., ENOD40 gene in Lotus japonicus. Are the two different ENOD40 genes differentially expressed?, (1998)

[7]

Handberg K., Stougaard J., Lotus japonicus, an autogamous, diploid legume species for classical and molecular genetics, Plant J, 2, pp. 487-496, (1992)

[8]

Heidstra R., Bisseling T., Nod factor-induced host responses and mechanisms of Nod factor perception, New Phytol, 133, pp. 25-43, (1996)

[9]

Hogetsu T., Detection of hemicelluloses specific to the cell wall of tracheary elements and phloem cells by fluorescein-conjugated lectins, Protoplasma, 156, pp. 67-73, (1990)

[10]

Imaizumi-Anraku H., Kawaguchi M., Koiwa H., Akao S., Syono K., Two ineffective-nodulating mutants of Lotus japonicus - Different phenotypes caused by the blockage of endocytotic bacterial release and nodule maturation, Plant Cell Physiol, 38, pp. 871-881, (1997)

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