Pseudoclavibacter-like subcutaneous infection: A case report

被引：10

作者：

Lemaitre F. ^{[1
]}

Stein A. ^{[2
]}

Raoult D. ^{[1
,3
]}

Drancourt M. ^{[1
,3
]}

机构：

[1] Pôle des Maladies Infectieuses, Fédération de Microbiologie Clinique, Hôpital de la Timone, 13005 Marseille, rue Saint-Pierre

[2] Pôle des Maladies Infectieuses, Service de Maladies Infectieuses et Tropicales, Hôpital de la Conception, 13005 Marseille, boulevard Baille

[3] Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Faculté de Médecine, Université de la Méditerranée, F-13385 Marseille cedex 5

来源：

Journal of Medical Case Reports | / 5卷 / 1期

关键词：

16S rRNA gene; identification; MALDI-TOF; Pseudoclavibacter; skin infection;

D O I：

10.1186/1752-1947-5-468

中图分类号：

学科分类号：

摘要：

Background: Arthrobacter-like organisms, including Pseudoclavibacter organisms, have rarely been documented as being responsible for infection in humans. Case presentation. An 81-year-old French man developed a subcutaneous infection despite antibiotic treatment combining clindamycin and metronidazole for chronic wound infection. A skin biopsy showed numerous polymorphonuclear cells and no bacteria, but a subcutaneous swab yielded numerous polymorphonuclear cells, a few Gram-positive cocci, Gram-negative cocci, and Gram-positive rods. The Gram-positive rod sequence exhibited 99% sequence similarity with uncultured Pseudoclavibacter sp. [GenBank:EF419350] and 99% sequence similarity with uncultured Pseudoclavibacter sp. [GenBank:EF419347]. The genetic data and unique peptide profile of this Pseudoclavibacter-like isolate, determined by matrix-assisted laser desorption ionization-time of flight mass spectrometry, underscored its uniqueness. Conclusions: Pseudoclavibacter-like organisms are identifiable in cutaneous and subcutaneous infections in humans. © 2011 Lemaitre et al; licensee BioMed Central Ltd.

引用

共 10 条

[1]

Manaia C.M., Nogales B., Weiss N., Nunes O.C., Gulosibacter molinativorax gen. nov., a molinate-degrading bacterium, and classification of 'Brevibacterium helvolum' DSM 20419 as Pseudoclavibacter helvolus gen. nov., sp. nov, International Journal of Systematic and Evolutionary Microbiology, 54, 3, pp. 783-789, (2004)

[2]

Mages I.S., Frodl R., Bernard K.A., Funke G., Identities of Arthrobacter spp. and Arthrobacter-like bacteria encountered in human clinical specimens, J Clin Microbiol, 46, pp. 2980-2986, (2008)

[3]

Martinez-Martinez L., Ortega M.C., Suarez A.I., Comparison of E-test with broth microdilution and disk diffusion for susceptibility testing of coryneform bacteria, J Clin Microbiol, 33, pp. 1318-1321, (1995)

[4]

Drancourt M., Berger P., Raoult D., Systematic 16S rRNA Gene Sequencing of Atypical Clinical Isolates Identified 27 New Bacterial Species Associated with Humans, Journal of Clinical Microbiology, 42, 5, pp. 2197-2202, (2004)

[5]

Seng P., Drancourt M., Gouriet F., La Scola B., Fournier P.E., Rolain J.M., Raoult D., Ongoing revolution in bacteriology: Routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry, Clin Infect Dis, 49, pp. 543-551, (2009)

[6]

Stackebrandt E., Ebers J., Taxonomic parameters revisited: Tarnished gold standards, Microbiol Today, 33, pp. 152-155, (2006)

[7]

Janda J.M., Abbott S.L., 16S rRNA gene sequencing for bacterial identification in the diagnostic laboratory: Pluses, perils, and pitfalls, Journal of Clinical Microbiology, 45, 9, pp. 2761-2764, (2007)

[8]

Medini D., Serruto D., Parkhill J., Relman D.A., Donati C., Moxon R., Falkow S., Rappuoli R., Microbiology in the post-genomic era, Nature Reviews Microbiology, 6, 6, pp. 419-430, (2008)

[9]

Hugenholtz P., Tyson G.W., Metagenomics, Nature, 455, pp. 481-483, (2008)

[10]

Gao Z., Tseng C.H., Strober B.E., Pei Z., Blaser M.J., Substantial alterations of the cutaneous bacterial biota in psoriatic lesions, PLoS One, 3, (2008)

← 1 →