Evaluation of CD44 and CD44v6 in colorectal carcinoma patients: Soluble forms in relation to tumor tissue expression and metastasis

被引：23

作者：

Amirghofran Z. ^{[1
,2
]}

Jalali S.A. ^{[1
]}

Hosseini S.V. ^{[3
]}

Vasei M. ^{[4
]}

Sabayan B. ^{[3
]}

Ghaderi A. ^{[1
,5
]}

机构：

[1] Immunology Department, Medical School, Shiraz University of Medical Science

[2] Autoimmune Disease Research Center, Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz

[3] Department of Surgery and Gastroentrohepatology, Shiraz University of Medical Sciences, Nemazi Hospital, Shiraz

[4] Department of Pathology, Shiraz University of Medical Sciences, Shiraz

[5] Cancer Research Institute, Shiraz University of Medical Sciences, Shiraz

来源：

Journal of Gastrointestinal Cancer | 2008年 / 39卷 / 1-4期

关键词：

CD44; CD44v6; Colorectal cancer;

D O I：

10.1007/s12029-009-9062-2

中图分类号：

学科分类号：

摘要：

Introduction: It has been shown that CD44 may be associated with poor prognosis in various human malignancies. This study was designed to investigate and compare the prognostic relevance and clinical value of soluble forms of CD44 and its variant v6 (CD44v6) and their tissue expression in colorectal carcinoma. Materials and methods: Serum levels of pre- and postoperative CD44 and CD44v6 molecules were evaluated in 37 colorectal cancer patients and compared to healthy individuals. Results and discussion: The serum levels of soluble CD44 and CD44v6 showed no significant decrease after surgical resection of the tumor (p=0.5). Both CD44 and CD44v6 serum levels either before or after surgery were significantly higher in patients than in normal individuals (p<0.001). The serum level of CD44v6 molecule was higher in patients with lymph node metastasis than other patients (p=0.05) implying the role of this molecule in tumor progression. Immunohistochemical staining showed expression of CD44 in 14.3% and CD44v6 in 42.9% of the tumor samples. The expression of CD44v6 was associated with metastatic involvement of lymph nodes (p=0.03). CD44v6 expression was positively correlated with its level in the serum of patients (p=0.04). Conclusions: Results of this study showed that CD44v6 expression level either in the soluble form or in the cell membrane is associated with tumor metastasis indicating the importance of this molecule in progression of colorectal carcinoma. The serum levels of soluble CD44 as well as CD44v6 might be useful markers for tumor screening. © 2009 Humana Press Inc.

引用

页码：73 / 78

页数：5

共 27 条

[1]

Hosseini S.V., Izadpanah A., Yarmohammadi H., Epidemiological changes in colorectal cancern in Shiraz, Iran: 1980-2000, ANZ Journal of Surgery, 74, 7, pp. 547-549, (2004)

[2]

Orr F.W., Wang H.H., Tumor cell interactions with the microvasculature: A rate-limiting step in metastasis, Surg Oncol Clin N Am, 10, pp. 357-381, (2001)

[3]

Iiizumi M., Mohinta S., Bandyopadhyay S., Watabe K., Tumor-endothelial cell interactions: Therapeutic potential, Microvascular Research, 74, 2-3, pp. 114-120, (2007)

[4]

Dalchau R., Kirkley J., Fabre J.W., Monoclonal anti-body to a human leukocyte-specific membrane glyco-protein probably homologous to the leukocyte-common (L-C) antigen of the rat, Eur J Immunol, 10, pp. 737-744, (1980)

[5]

Dianzani U., Malavasi F., Lymphocyte adhesion to endothelium, Crit Rev Immunol, 15, pp. 167-200, (1995)

[6]

Screaton G.R., Bell M.V., Jackson D.G., Cornelis F.B., Gerth U., Bell J.I., Genomic structure of DNA encoding the lymphocyte homing receptor CD44 reveals at least 12 alternatively spliced exons, Proceedings of the National Academy of Sciences of the United States of America, 89, 24, pp. 12160-12164, (1992)

[7]

Gunthert U., Hofmann M., Rudy W., Et al., A new variant of glycoprotein CD44 confers metastatic potential to rat carcinoma cells, Cell, 65, pp. 13-24, (1991)

[8]

Iida N., Bourguignon L.Y.W., New CD44 splice variants associated with human breast cancers, J Cell Physiol, 162, pp. 127-133, (1995)

[9]

Manten-Horst E., Danen E.H., Smit L., Et al., Expression of CD44 splice variants in human cutaneous melanoma and melanoma cell lines is related to tumor progression and metastatic potential, Int J Cancer Pred Oncol, 64, pp. 182-188, (1995)

[10]

Picker L.J., De Los Toyos J., Telen M.J., Et al., Monoclonal antibodies against the CD44 [in(lu)-related p80] and pgp-1 antigens in man recognize the Hermes class of lymphocyte homing receptors, J Immunol, 142, pp. 2046-2051, (1989)

← 1 2 3 →