Modification of collagen formation using supplemented mesh materials

被引：23

作者：

Junge K. ^{[1
,3
,4
]}

Rosch R. ^{[1
,3
,4
]}

Anurov M. ^{[4
]}

Titkova S. ^{[4
]}

Öttinger A. ^{[4
]}

Klinge U. ^{[1
,2
,3
,4
]}

Schumpelick V. ^{[1
,4
]}

机构：

[1] Department of Surgery, Technical University of Aachen, 52074 Aachen

[2] IZKF BIOMAT, Technical University of Aachen, 52074 Aachen

[3] German Centre of Excellence for Biomaterial and Implant Pathology, Technical University of Aachen, 52074 Aachen

[4] Joint Institute for Surgical Research, Technical University of Aachen, 52074 Aachen

来源：

Hernia | 2006年 / 10卷 / 6期

关键词：

Ascorbic acid; Collagen; Doxycycline; Hyaluronic acid; Mesh; TGF-beta; 3; Zinc-hydrogene-aspartate;

D O I：

10.1007/s10029-006-0148-5

中图分类号：

学科分类号：

摘要：

Background: Formation of recurrent inguinal and incisional hernia shows an underlying defect in the wound healing process. Even following mesh repair an altered collagen formation and insufficient mesh integration has been found as main reason for recurrences. Therefore the development of bioactive mesh materials to achieve a local modification of the scar formation to improve patients outcome is advisable. Methods: Thirty-sixmale Wistar rats were used within this study. A Mersilene® mesh sample was implanted after midline skin incision and subcutaneous preparation. Before implantation mesh samples were incubated for 30 minutes with either one of the following agents: doxycycline, TGF-beta 3, zinc-hydrogeneaspartate, ascorbic acid, hyaluronic acid. Incubation with a physiologic 0.9 % NaCl solution served as control. Seven and 90 days after mesh implantation 3 animals from each group (n = 6) were sacrificed for morphological observations. Collagen quantity and quality was analyzed measuring the collagen/ protein as well as the collagen type I/III ratio. Results: Following animplantation interval of 90 days supplementation with doxycycline (39.3 ± 7.0 μg/mg) and hyaluronic acid (34.4 ± 5.8 μg/mg) were found to have a significantly increased collagen/protein ratio compared to implantation of the pure Mersilene® mesh samples (28.3 ± 1.9 μg/mg). Furthermore, an overall increase of the collagen type I/III ratio was found in all groups indicating scar maturation over time. However, no significant differences were found after 7 and 90 days of implantation comparing collagen type I/III ratio of supplemented mesh samples and control group. Conclusions: In summary, we found an influence of supplemented mesh materials on collagen deposition. However, the investigated bioactive agents with reported influence on wound healing were not associated with an improved quality in scar formation. © Springer-Verlag 2006.

引用

页码：492 / 497

页数：5

共 39 条

[1]

Agren M.S., Studies on zinc in wound healing, Acta Derm Venereol Suppl (Stockh), 154, pp. 1-36, (1990)

[2]

Bellon J.M., Bajo A., Ga-Honduvilla N., Gimeno M.J., Pascual G., Guerrero A., Et al., Fibroblasts from the transversalis fascia of young patients with direct inguinal hernias show constitutive MMP-2 overexpression, Ann Surg, 233, 2, pp. 287-291, (2001)

[3]

Bellon J.M., Bujan J., Honduvilla N.G., Jurado F., Gimeno M.J., Turnay J., Et al., Study of biochemical substrate and role of metalloproteinases in fascia transversalis from hernial processes, Eur J Clin Invest, 27, 6, pp. 510-516, (1997)

[4]

Birk D.E., Mayne R., Localization of collagen types I, III and V during tendon development. Changes in collagen types I and III are correlated with changes in fibril diameter, Eur J Cell Biol, 72, 4, pp. 352-361, (1997)

[5]

Dubay D.A., Wang X., Kuhn M.A., Robson M.C., Franz M.G., The prevention of incisional hernia formation using a delayed-release polymer of basic fibroblast growth factor, Ann Surg, 240, 1, pp. 179-186, (2004)

[6]

[7]

Farris P.K., Topical vitamin C: A useful agent for treating photoaging and other dermatologic conditions, Dermatol Surg, 31, 7 PART 2, pp. 814-817, (2005)

[8]

Friedman D.W., Boyd C.D., Mackenzie J.W., Norton P., Olson R.M., Deak S.B., Regulation of collagen gene expression in keloids and hypertrophic scars, J Surg Res, 55, 2, pp. 214-222, (1993)

[9]

Friedman D.W., Boyd C.D., Norton P., Greco R.S., Boyarsky A.H., Mackenzie J.W., Et al., Increases in type III collagen gene expression and protein synthesis in patients with inguinal hernias, Ann Surg, 218, 6, pp. 754-760, (1993)

[10]

Guo Y., Xie C., Rodriguez R.M., Light R.W., Factors related to recurrence of spontaneous pneumothorax, Respirology, 10, 3, pp. 378-384, (2005)

← 1 2 3 4 →