Genomic Structure of a Geminivirus in the Genus Begomovirus from Yellow Vein-affected Eupatorium makinor

A virus from yellow vein-affected Eupatorium makinoi was tentatively designated as Eupatorium-infecting geminivirus (EuGV) on the basis of whitefly transmissibility, electron microscopic observation of geminate particles in sap and symptomatology. EuGV-speciflc DNA fragments were obtained by polymerase chain reaction (PCR). Based on the restriction analysis of the PCR products, EuGV was suggested to have a monopartite genome. We determined the putative, complete nucleotide sequence of the EuGV genome which is comprised of 2766 nucleotides. The EuGV sequence had two virus sense open reading frames (ORF)(V1, V2), four complementary sense ORFs (C1–C4) and a non-coding region termed the intergenic region. This genome structure is quite similar to other monopartite begomoviruses already reported. The nucleotide and amino acid sequences were compared with other begomoviruses. The results supported the conclusion that EuGV is distinct and divergent from other begomoviruses, whereas potential sequence motifs reported in other geminiviruses are well conserved.