Assessment of genetic diversity of tea (Camellia sinensis (L.) O. Kuntze) by inter-simple sequence repeat polymerase chain reaction

被引：46

作者：

Mondal T.K. ^{[1
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机构：

[1] Biotechnology Laboratory, Research and Development Department, Tata Tea Limited, Munnar, Kerala

[2] Ctr. Advance Stud. Tea Sci./Technol., Dept. of Plantation Crops/Proc., Uttar Banga Krishi Viswavidyalaya, Pin: 736165, West Bengal, P.O: Pundibari, Dist Cooch Behar

来源：

Euphytica | 2002年 / 128卷 / 3期

关键词：

Camellia sinensis; ISSR-PCR; Microsatellite DNA; Repetitive DNA; Tea;

D O I：

10.1023/A:1021212419811

中图分类号：

学科分类号：

摘要：

Twenty-five diverse tea (Camellia sinensis (L.) O. Kuntze) cultivars were analyzed using the simple sequence repeat anchored polymorearse chain reaction (SSR-anchored PCR) or Inter SSR-PCR (ISSR). Out of the 45 primers 12 were chosen for final study. These amplified a total of 130 bands out of which 108 (84%) were polymorphic. A dendrogram was constructed using UPGMA method revealed three distinct clusters of Cambod, Assam and China type, which concur with the known taxonomical classification of tea. These results suggest that the ISSR-PCR method is potentially useful for genetic fingerprinting and molecular taxonomic classification of tea genotypes.

引用

页码：307 / 315

页数：8

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