A simple colorimetric method for determination of hydrogen peroxide in plant tissues

被引：14

作者：

Biyan Zhou

Jihua Wang

Zhenfei Guo

Huoquan Tan

Xiaochuan Zhu

机构：

[1] South China Agricultural University,College of Life Science

[2] South China Agricultural University,Physiological Laboratory for South China Fruits, College of Horticulture

来源：

Plant Growth Regulation | 2006年 / 49卷

关键词：

4-aminoantipyrine; Hydrogen peroxide; Peroxidase; Plant tissue;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A simple colorimetric method for determination of hydrogen peroxide in plant materials is described. The method is based on hydrogen peroxide producing a stable red product in reaction with 4-aminoantipyrine and phenol in the presence of peroxidase. Plant tissues was ground with trichloroacetic acid (5% w/v) and extracts were adjusted to pH 8.4 with ammonia solution. Activated charcoal was added to the homogenate to remove pigments, antioxidants and other interfering substances. The colorimetric reagent (pH 5.6) consisted of 4-aminoantipyrine, phenol, and peroxidase. With this method, we have determined the hydrogen peroxide concentration in leaves of eight species which ranged from 0.2 to 0.8 µmol g−1 FW. Changes in hydrogen peroxide concentration of Stylosanthes guianensis in response to heat stress are also analyzed using this method.

引用

页码：113 / 118

页数：5

共 75 条

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