Extra divisions and nuclei fusions in microspores from Brassica allohexaploid (AABBCC) × Orychophragmus violaceus hybrids

被引:2
作者
Xian-Hong Ge
Zai-Yun Li
机构
[1] National Center of Oil Crop Improvement (Wuhan),National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding Technology
[2] Huazhong Agricultural University,undefined
来源
Plant Cell Reports | 2006年 / 25卷
关键词
allohexaploid; Cell cycle; Cell fusion; Meiosis; Microspore; Multi-polar division;
D O I
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中图分类号
学科分类号
摘要
Abnormal meiosis and microspore development and related defective mutants have often been reported in plants and wide hybrids. Here extra divisions and nuclei fusions were observed to occur in microspore nuclei of partial hybrids between synthetic Brassica hexaploid (2n=54, AABBCC) and another crucifer Orychophragmus violaceus (2n=24). Abnormal spindle were formed and chromosomes were separated into several nuclei of variable sizes after bi-, or multi-polar divisions in the four cells of tetrads. As a consequence, more than eight mini-microspores of different sizes were produced by one tetrad. Genomic in situ hybridization results indicated that no chromosome replication occurred during such divisions. In some tetrads, the four nuclei were fused to form one large cell with increased chromosome number. The extra divisions or fusions appeared only in some flower buds of one plant, some anthers in the same buds, or even in individual cells of tetrads. The possible mechanisms behind these cytological phenomena are discussed.
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