Probable interaction between S100A7 and E-FABP in the cytosol of human keratinocytes from psoriatic scales

被引：6

作者：

Gerry Hagens

Karen Roulin

Raymonde Hotz

Jean-Hilaire Saurat

Ulf Hellman

Georges Siegenthaler

机构：

[1] University Hospital,Department of Dermatology and DHURDV

[2] Ludwig Institute for Cancer Research,undefined

来源：

Molecular and Cellular Biochemistry | 1999年 / 192卷

关键词：

psoriasis; calcium-binding protein; protein-protein interaction;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The overexpression of E-FABP and S100A7 in lesional psoriatic skin suggests a possible link with this hyperproliferative skin disease. In order to investigate a role for the proteins in this disease, the purifications for both proteins were re-analyzed. Moreover, a specific antiserum directed against purified human S100A7 was generated. By SDS-PAGE immunoblotting we show that E-FABP and S100A7 are expressed in cultured human differentiating keratinocytes and confirm their overexpression in psoriatic scales. Gel filtration and non-denaturing PAGE revealed that S100A7 co-purified with E-FABP, indicating an association between the two proteins. Ion-exchange chromatography resulted in the dissociation of the complex. Finally, immunoprecipitations using antiserum against E-FABP revealed that S100A7 co-immunoprecipitated with E-FABP from protein extracts of psoriatic scales. These data indicate that E-FABP and S100A7 might form a complex in the cytosol of human keratinocytes.

引用

页码：123 / 128

页数：5

共 88 条

[1]

Elias PM(1991)Structural and biochemical correlates of the epidermal permeability barrier Adv Lipid Res 24 1-26

[2]

Menon GK(1994)Stratum corneum moisturization at the molecular level J Invest Dermatol 103 731-740

[3]

Rawlings AV(1993)Characterization and expression of a novel human fatty acid-binding protein: The epidermal type (E-FABP) Biochem Biophys Res Commun 190 482-487

[4]

Scott IR(1994)Purification and characterization of the human epidermal fatty acid-binding protein: Localization during epidermal differentiation Biochem J 302 363-371

[5]

Harding CR(1992) and J Invest Dermatol 99 299-305

[6]

Browser PA(1997)Molecular cloning and expression of a novel keratinocyte protein [psoriasis-associated fatty acid binding protein (PA-FABP)] that is highly up-regulated in psoriatic skin and that shares similarity to fatty acid-binding proteins J Biol Chem 272 9371-9377

[7]

Siegenthaler G(1997)A heterocomplex formed by the calciumbinding proteins MRP8 (S100A8) and MRP14 (S100A9) binds unsaturated fatty acids Circ Res 81 297-303

[8]

Hotz R(1987)Endothelial cells of the human microvasculature express epidermal fatty acid-binding protein (E-FABP) Dermatologica 174 209-213

[9]

Chatellard-Gruaz D(1989)Eicosanoids in psoriasis-15–HETE on the stage J Invest Dermatol 92 837-841

[10]

Jaconi S(1988)Eicosanoids in acute and chronic psoriatic lesions: Leukotriene B4, but not 12–hydroxy-eicosatetraenoic acid, is present in biologically active amounts in acute guttate lesions J Invest Dermatol 91 446-450

← 1 2 3 4 5 6 7 8 9 →