Analysis of the promoter activity of late embryogenesis abundant protein genes in barley seedlings under conditions of water deficit

被引：33

作者：

Xiao F.-H. ^{[1
,2
]}

Xue G.-P. ^{[1
]}

机构：

[1] CSIRO Plant Industry, Indooroopilly, QLD 4068

[2] Department of Biotechnology, Yunnan Agricultural University, Kunming, Yunnan

来源：

Plant Cell Reports | 2001年 / 20卷 / 7期

关键词：

Abscisic acid; Barley; Drought; Lea promoter; Transient expression;

D O I：

10.1007/s002990100384

中图分类号：

学科分类号：

摘要：

Promoters of the late embryogenesis abundant protein genes, HVA1s, Dhn8s and Dhn4s from barley and wsi18j and rab16Bj from rice, were analysed in barley seedlings to assess their strength and timing of induction under water deficit conditions using a transient expression system. Of the drought-inducible promoters, Dhn4s exhibited the highest activity, followed by HVA1s, wsi18j and rab16Bj. Dehydration-induced β-glucuronidase expression levels driven by the Dhn4s, HVA1s and wsi18j promoters were higher than or comparable to that induced by a strong constitutive rice Act1 promoter. The expression patterns of wsi18j and Dhn8s differed from those of their homologous genes (wsi18 and Dhn8): the wsi18j promoter was strongly induced by abscisic acid, while the Dhn8s promoter was highly active in hydrated seedlings. HVA1s- and wsi18j-driven green fluorescent protein expression was induced within 1 h of drying under water deficit stress, while Dhn4s promoter activity was not detectable until 3 h, thus showing a difference in the timing of induction.

引用

页码：667 / 673

页数：6

共 22 条

[1]

Bajaj S., Targolli J., Liu L.-F., Ho T.-H.D., Wu R., Transgenic approaches to increase dehydration-stress tolerance in plants, Mol Breed, 5, pp. 493-503, (1999)

[2]

Bray E.A., Plant responses to water deficit, Trends Plant Sci, 2, pp. 48-54, (1997)

[3]

Chandler P.M., Robertson M., Gene expression regulated by abscisic acid and its relation to stress tolerance, Annu Rev Plant Physiol Plant Mol Biol, 45, pp. 113-141, (1994)

[4]

Choi D.-W., Zhu B., Close T.J., The barley (Hordeum vulgare L.) dehydrin multigene family: Sequences, allele types, chromosome assignments, and expression characteristics of 11 Dhn genes of cv. Dicktoo, Theor Appl Genet, 98, pp. 1234-1247, (1999)

[5]

Grossi M., Cattivelli L., Terzi V., Stanca A.M., Modification of gene expression induced by ABA, in relation to drought and cold stress in barley shoots, Plant Physiol Biochem, 30, pp. 97-103, (1992)

[6]

Grossi M., Gulli M., Stanca A.M., Cattivelli L., Characterisation of two barley genes that respond rapidly to dehydration stress, Plant Sci, 105, pp. 71-80, (1995)

[7]

Holmberg N., Bulow L., Improving stress tolerance in plants by gene transfer, Trends Plant Sci, 3, pp. 61-66, (1998)

[8]

Hong B., Barg R., Ho T.-H.D., Developmental and organ-specific expression of an ABA- and stress-induced protein in barley, Plant Mol Biol, 18, pp. 663-674, (1992)

[9]

Jefferson R.A., Assaying chimeric genes in plants: The Gus gene fusion system, Plant Mol Biol Rep, 5, pp. 387-405, (1987)

[10]

Joshee N., Kisaka H., Kitagawa Y., Isolation and characterization of a water stress-specific genomic gene, pwsi 18, from rice, Plant Cell Physiol, 39, pp. 64-72, (1998)

← 1 2 3 →