Cytoprotection against thermal injury: Evaluation of herbimycin A by cell viability and cDNA arrays

被引：4

作者：

Dihn H.K. ^{[1
,2
]}

Stavchansky S. ^{[1
]}

Schuschereba S.T. ^{[2
]}

Stuck B.E. ^{[2
]}

Bowman P.D. ^{[1
,3
]}

机构：

[1] Division of Pharmaceutics, The University of Texas, Austin, TX

[2] US Army Medical Research Detachment, Brooks AFB, TX

[3] US Army Inst. of Surgical Research, Fort Sam Houston, TX 78234-6315

来源：

The Pharmacogenomics Journal | 2002年 / 2卷 / 5期

关键词：

Gene expression; Heat shock proteins; Herbimycin A; Stress response; Thermal Injury; Thermotolerance;

D O I：

10.1038/sj.tpj.6500120

中图分类号：

学科分类号：

摘要：

Herbimycin A (HA), a known inducer of the heat shock response, was investigated for its ability to increase survival of a human cell line following thermal injury. Its effect on transcriptional activity was also assessed with cDNA arrays to provide new targets for cytoprotection. Pretreatment with at least 0.75 μg/ml HA significantly increased the fraction of cells surviving thermal injury by up to 50% (based on 8s exposure) compared to untreated controls. HA also significantly induced transcription of mRNA for HSP90 and HSP70, and protein production for HSP40 and HSP70. Gene expression profiling demonstrated that the most highly elevated genes included growth factors and transcription factors, while prominently suppressed genes included transcription factors and kinases. These results suggest that cytoprotection may be due to the contribution of the products of a significant number of genes in addition to the classic stress response genes, suggesting that modulation of these genes might induce thermotolerance and amelioration of thermal injury.

引用

页码：318 / 326

页数：8

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