Ex vivo effects of lysine clonixinate on cyclooxygenases in rat lung and stomach preparations

被引：5

作者：

Franchi A.M. ^{[1
]}

Di Girolamo G. ^{[2
]}

De Lossantos A.R. ^{[3
]}

Marti M.L. ^{[3
]}

Gimeno M.A.F. ^{[1
]}

机构：

[1] Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Cientificas y Técnicas (CONICET)

[2] Segunda Cátedra de Farmacologia, Facultad de Medicina, Universidad de Buenos Aires

[3] Hospital de Clinicas José de San Martin, Facultad de Medicina, Universidad de Buenos Aires

来源：

InflammoPharmacology | 1999年 / 7卷 / 4期

关键词：

Anti-inflammatory drug; Cyclooxygenase; Lysine clonixinate;

D O I：

10.1007/s10787-999-0033-9

中图分类号：

学科分类号：

摘要：

Lysine clonixinate (LC) is an anti-inflammatory, anti-pyretic and analgesic drug with minor digestive side effects, which might suggest a weak COX-1 inhibitor. The aim of this study focused on ex vivo effects of LC 40 mg/kg ip and indomethacin (INDO) 10 mg/kg ip in lung and stomach preparations of control rats and LPS-treated rats (5 mg/kg ip). The non-steroidal antiinflammatory drugs were administered concomitantly, following three hours and before one, two or three hours of LPS treatment. Tissues were weighed and incubated in 2 ml of Kress Ringer Bicarbonate buffer containing glucose (11 mM) under an atmosphere of 95% oxygen and 5% CO2. Approximately 200 mg of tissue were used for each determination; 0.25 μCi of 14C-arachidonic acid was added to each tube and the tissues were incubated for 60 min. Prostanoids were extracted from the incubation medium and separated by TLC. Results were expressed as a percentage of the total radioactivity of the plates (% of cpm on plate/100 mg ww). It was found that LC animals that were not given LPS did not modify the synthesis of PGE2; in lung and stomach tissues showing that did not inhibit COX-1 activity. However, LC inhibited clearly the synthesis of PGE2 in both preparations obtained from LPS-treated animals. The inhibition was shown when the rats were treated concomitantly, 3 h after or 1 or 2 h before the injection of LPS.

引用

页码：401 / 411

页数：10

共 16 条

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