A Rapid and Efficient DNA Minipreparation Suitable for Screening Transgenic Plants

被引：70

作者：

Lin R.-C. ^{[1
]}

Ding Z.-S. ^{[1
]}

Li L.-B. ^{[1
]}

Kuang T.-Y. ^{[1
]}

机构：

[1] Photosynthesis Research Center, Institute of Botany, Chinese Academy of Sciences

来源：

Plant Molecular Biology Reporter | 2001年 / 19卷 / 4期

关键词：

DNA extraction; SDS; Transgenic plants;

D O I：

10.1007/BF02772839

中图分类号：

学科分类号：

摘要：

We present a modified method for DNA minipreparation suitable for large-scale screening of transgenic plants. The method is rapid and efficient - one person can prepare DNA from approximately 50 samples per day. The average yield was about 40 μg DNA per 100 mg of fresh tissue, and the A260/A280 was 1.89-2.03. The total DNA extracted by this method could be used for PCR, restriction enzyme digestion, and Southern blotting.

引用

页码：379a / 379e

共 11 条

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