Cytotoxicity and arecoline mechanisms in human gingival fibroblasts in vitro

被引：45

作者：

Chang Y.-C. ^{[1
]}

Hu C.-C. ^{[2
]}

Lii C.-K. ^{[3
]}

Tai K.-W. ^{[4
]}

Yang S.-H. ^{[4
]}

Chou M.-Y. ^{[1
]}

机构：

[1] Institute of Stomatology, Chung Shan Medical and Dental College, Taichung, 23 Section 1, Taichung-Kang Rd

[2] Department of Biochemistry, Chung Shan Medical and Dental College, Taichung

[3] Institute of Nutritional Science, Chung Shan Medical and Dental College, Taichung

[4] School of Dentistry, Chung Shan Medical and Dental College, Taichung

来源：

Clinical Oral Investigations | 2001年 / 5卷 / 1期

关键词：

Keywords Arecoline; Fibroblasts; Thiols depletion; Mitochondrial function; Cell cycle;

D O I：

10.1007/s007840000085

中图分类号：

学科分类号：

摘要：

Betel nut chewing, like cigarette smoking, is a popular oral habit which impinges on the daily lives of a population of approximately 200 million. People who chew betel nuts have a higher prevalence of periodontal diseases than those who do not. Many of the undesirable effects of betel nuts have been attributed to arecoline, a major component of the particular alkaloid in betel nuts. In this in vitro study, we have focused on the effects of arecoline and the role it could play in periodontal breakdown via its direct effects on human gingival fibroblasts. Human gingival fibroblasts were derived from three healthy individuals undergoing crown-lengthening procedures. We found that arecoline is cytotoxic to human gingival fibroblasts at a concentration higher than 50 micrograms/ml by depleting intracellular thiols and inhibiting mitochondrial activity (P < 0.05). In addition, the cells displayed a marked arrest at G2/M phase in a dose-dependent manner. Repeated and long-term exposure to arecoline could impair the gingival fibroblast functions. As they are cytotoxic, the use of betel nut products in conjunction with periodontal therapy may interfere with optimal healing and/or lead to further periodontal breakdown.

引用

页码：51 / 56

页数：5