Transcriptional profile of Toxoplasma gondii-infected human fibroblasts as revealed by gene-array hybridization

被引：41

作者：

Gail M. ^{[1
]}

Gross U. ^{[1
]}

Bohne W. ^{[1
]}

机构：

[1] Department of Bacteriology, University of Göttingen, 37075 Göttingen

来源：

Molecular Genetics and Genomics | 2001年 / 265卷 / 5期

关键词：

Gene array; Gene expression; Host cell response; RT-PCR; Toxoplasma gondii;

D O I：

10.1007/s004380100487

中图分类号：

学科分类号：

摘要：

To investigate the host-cell response to infection with the obligate intracellular pathogen Toxoplasma gondii, the transcriptional profiles of infected and uninfected human fibroblasts (HFF) were determined by hybridization to gene arrays representing nearly 600 genes. Transcripts that displayed a greater than five-fold increase in level relative to uninfected controls were also examined by RT-PCR and Northern analysis, resulting in the identification of 13 genes that were strongly upregulated after infection with T. gondii. Comparisons with the transcriptional profiles of fibroblasts infected with Salmonella typhimurium and Chlamydia trachomatis allowed the identification of genes which are specifically induced in T. gondii-infected cells. While most of the upregulated genes were induced on infection with all three pathogens, the genes for the transferrin receptor and MacMARCKS were up-regulated in Toxoplasma-infected fibroblasts only. Expression of the transferrin receptor protein was examined by Western analysis and found to be specifically elevated in Toxoplasma-infected fibroblasts. Genes which are specifically induced in T. gondii-infected cells are particularly interesting for further studies, since they might be used to dissect specific interactions of this pathogenic parasite with its host cell.

引用

页码：905 / 912

页数：7

共 25 条

[1]

Bliss S.K., Marshall A.J., Zhang Y., Denkers E.Y., Human polymorphonuclear leukocytes produce IL-12, TNF-alpha, and the chemokines macrophage-inflammatory protein-1 alpha and -1 beta in response to Toxoplasma gondii antigens, J Immunol, 162, pp. 7369-7375, (1999)

[2]

Bonhomme A., Bouchot A., Pezzella N., Gomez J., Le Moal H., Pinon J.M., Signaling during the invasion of host ceils by Toxoplasma gondii, FEMS Microbiol Rev, 23, pp. 551-561, (1999)

[3]

Denney C.F., Eckmann L., Reed S.L., Chemokine secretion of human cells in response to Toxoplasma gondii infection, Infect Immun, 67, pp. 1547-1552, (1999)

[4]

Dobrowolski J., Sibley L.D., The role of the cytoskeleton in host cell invasion by Toxoplasmo gondii, Behring Inst Mitt, 99, pp. 90-96, (1997)

[5]

Dubremetz J.F., Garcia-Reguet N., Conseil V., Fourmaux M.N., Apical organelles and host-cell invasion by Apicomplexa, Int J Parasitol, 28, pp. 1007-1013, (1998)

[6]

Fischer H.G., Nitzgen B., Reichmann G., Hadding U., Cytokine responses induced by Toxoplasma gondii in astrocytes and microglial cells, Eur J Immunol, 27, pp. 1539-1548, (1997)

[7]

Galan J.E., Interaction of Salmonella with host cells through the centisome 63 type III secretion system, Curr Opin Microbiol, 2, pp. 46-50, (1999)

[8]

Garcia-Del Portillo F., Finlay B.B., The varied lifestyles of intracellular pathogens within eukaryotic vacuolar compartments, Trends Microbiol, 3, pp. 373-380, (1995)

[9]

Goebel S., Luder C.G., Gross U., Invasion by Toxoplasma gondii protects human-derived HL-60 cells from actinomycin D-induced apoptosis, Med Microbiol Immunol, 187, pp. 221-226, (1999)

[10]

Gomez-Marin J.E., Valere A., Bonhomme A., El'Btaouri H., Antonicelli F., Burlet H., Aubert D., Villena I., Guenounou M., Haye B., Pinon J.M., Interferon-gamma signal transduction during parasite infection: Modulation of MAP kinases in the infection of human monocyte cells (THP1) by Toxoplasma gondii, Parasite Immunol, 20, pp. 631-635, (1998)

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