Rapid colorimetric detection of proteins and bacteria using silver reduction/precipitation catalyzed by gold nanoparticles

被引：3

作者：

Betala P.A. ^{[1
]}

Appugounder S. ^{[2
]}

Chakraborty S. ^{[1
]}

Songprawat P. ^{[1
]}

Buttner W.J. ^{[2
]}

Perez-Luna V.H. ^{[1
]}

机构：

[1] Department of Chemical and Biological Engineering, Illinois Institute of Technology, Chicago, IL 60616

[2] Department of Biological, Chemical and Physical Sciences, Illinois Institute of Technology, Chicago

来源：

Sensing and Instrumentation for Food Quality and Safety | 2008年 / 2卷 / 01期

关键词：

Antibodies; Antigens; Colorimetric detection; Escherichia coli; Goldnano particles; Silver reduction;

D O I：

10.1007/s11694-007-9026-9

中图分类号：

学科分类号：

摘要：

A colorimetric immunoassay has been developed based on the reduction and precipitation of silver ions catalyzed by colloidal gold. This method was evaluated for a noncompetitive heterogeneous immunoassay to detect ovalbumin as a model protein and Escherichia coli as model bacterium. The influence of relevant experimental variables, including the reaction time of antigen with antibody, the dilution ratio of the colloidal gold-labeled protein, time allowed for silver reduction/precipitation reaction and other parameters were examined and optimized. This colorimetric method of detection is convenient, easy to use and economic. The presence of the target molecules or antigens can be visually inferred based on the color change that occurs in a short time without using major instrumentation. This method is widely amenable for detection of proteins, viruses and bacteria. © Springer Science+Business Media, LLC 2007.

引用

页码：34 / 42

页数：8

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