Enhancement of apparent resistance to ethanol in Lactobacillus hilgardii

被引:13
作者
Couto J.A. [1 ]
Pina C. [1 ]
Hogg T. [1 ]
机构
[1] Escola Superior de Biotecnologia, Univ. Católica Portuguesa, 4200 Porto, R. A. Bernardino Almeida
关键词
Lactobacillus; Butanol; Ethanol Tolerance; Ethanol Stress; Lethal Challenge;
D O I
10.1023/A:1018312714761
中图分类号
学科分类号
摘要
The survival of Lactobacillus hilgardii, a highly ethanol-tolerant organism, after an ethanol challenge at 25% (v/v) for 10 min, increased by several log cycles when cells, grown in the absence of ethanol, were pre-treated with 10% (v/v) ethanol, 15% (v/v) methanol or 2% (v/v) butanol for 4 h. A temperature upshift (25 to 40°C) before ethanol challenge demonstrated a similar enhancement of apparent resistance to ethanol. Ethanol shock enhanced apparent resistance to methanol, butanol and heat challenges. The addition of chloramphenicol to cells prior to any pre-treatment did not significantly diminish the increase in ethanol tolerance, suggesting that de novo protein synthesis is not required for induced tolerance organism.
引用
收藏
页码:487 / 490
页数:3
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