Luminol-enhanced chemiluminescence of rabbit polymorphonuclear leukocytes: The nature of oxidants that directly cause luminol oxidation

被引：4

作者：

Roshchupkin D.I. ^{[1
]}

Belakina N.S. ^{[2
]}

Murina M.A. ^{[2
]}

机构：

[1] Russian State Medical University

[2] Research Institute of Physicochemical Medicine

来源：

Biophysics | 2006年 / 51卷 / 1期

关键词：

Amino acid; Biogenic chloramines; Chemiluminescence; Dimethyl sulfoxide; Hydroxyl radical; Hypochlorite; Leukocyte; Luminol;

D O I：

10.1134/S000635090601012X

中图分类号：

学科分类号：

摘要：

In this study, we investigated the pathways (including the formation of hydroxyl radicals and chloramines) leading to luminol chemiluminescence induced by hypochlorite generated in a suspension of stimulated rabbit polymorphonuclear leukocytes. Chemiluminescence of leukocytes stimulated by phorbol myristate acetate, which was enhanced by luminol (0.02 mM), did not change in the presence of dimethyl sulfoxide at moderate concentrations (0.02-2.6 mM), under which the latter should manifest the specific ability to scavenge hydroxyl radicals. This indicates that stimulation of polymorphonuclear leukocytes is not accompanied by the generation of hydroxyl radicals with the involvement of superoxide anion and hypochlorite synthesized by myeloperoxidase. At high concentrations of dimethyl sulfoxide (260 mM), chemiluminescence markedly declined because dimethyl sulfoxide directly reacts with hypochlorite. The luminol emission intensity considerably increased after its addition to a suspension of leukocytes that were preliminarily stimulated for 10 min. This effect was caused by the accumulation of hydrogen peroxide rather than chloramines. Exogenous amino acids and taurine at high concentrations (3-15 mM) quench chemiluminescence. All these data indicate that chemiluminescence in the system studied is largely determined by the direct initial reaction of hypochlorite with luminol, the emission intensity increasing as a result of oxidation of luminol transformation products by hydrogen peroxide. © 2006 Nauka/Interperiodica.

引用

页码：79 / 86

页数：7

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