Transgenic Italian ryegrass (Lolium multiflorum) plants from microprojectile bombardment of embryogenic suspension cells

被引：44

作者：

Ye X. ^{[1
]}

Wang Z.-Y. ^{[1
,2
]}

Wu X. ^{[1
]}

Potrykus I. ^{[1
]}

Spangenberg G. ^{[1
,2
]}

机构：

[1] Institute for Plant Sciences, Swiss Fed. Institute of Technology

[2] Plant Sciences and Biotechnology, Agriculture Victoria, La Trobe University, Bundoora

来源：

Plant Cell Reports | 1997年 / 16卷 / 6期

关键词：

Forage and turf grasses; Italian ryegrass; Lolium multiflorum; Microprojectile bombardment; Transgenic plants;

D O I：

10.1007/BF01146777

中图分类号：

学科分类号：

摘要：

Transgenic forage-type Italian ryegrass (Lolium multiflorum Lam.) plants have been obtained by microprojectile bombardment of embryogenic suspension cells using a chimeric hygromycin phosphotransferase (hph) gene construct driven by rice Actl 5' regulatory sequences. Parameters for the bombardment of embryogenic suspension cultures with the particle inflow gun were partially optimized using transient expression assays of a chimeric β-glucuronidase (gusA) gene driven by the maize Ubi I promoter. Stably transformed clones were recovered with a selection scheme using hygromycin in liquid medium followed by a plate selection. Plants were regenerated from 33% of the hygromycin-resistant calli. The transgenic nature of the regenerated plants was demonstrated by Southern hybridization analysis. Expression of the transgene in transformed adult Italian ryegrass plants was confirmed by northern analysis and a hygromycin phosphotransferase enzyme assay.

引用

页码：379 / 384

页数：5

共 25 条

[1]

Asano Y., Ugaki M., Transgenic plants of Agrostis alba obtained by electroporation-mediated direct gene transfer into protoplasts, Plant Cell Rep, 13, pp. 243-246, (1994)

[2]

Bilang R., Iida S., Peterhans A., Potrykus I., Paszkowski J., The 3′-terminal region of the hygromycin-B-resistance gene is important for its activity in Escherichia coli and Nicotiana tabacum, Gene, 100, pp. 247-250, (1991)

[3]

Christensen A.H., Sharrock R.A., Quail P.H., Maize polyubiquitin genes: Structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation, Plant Mol Biol, 18, pp. 675-689, (1992)

[4]

Feinberg A.P., Vogelstein B., A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity, Anal Biochem, 132, pp. 6-13, (1983)

[5]

Finer J.J., Vain P., Jones M.W., McMullen M.D., Development of the particle inflow gun for DNA delivery to plant cells, Plant Cell Rep, 11, pp. 323-328, (1992)

[6]

Futterer J., Gisel A., Iglesias V., Kloti A., Kost B., Mittelsten Scheid O., Neuhaus G., Neuhaus-Url G., Schrott M., Shillito R., Spangenberg G., Wang Z.Y., Standard molecular techniques for the analysis of transgenic plants, Gene Transfer to Plants, pp. 215-262, (1995)

[7]

Ha S.B., Wu F.S., Thorne T.K., Transgenic turf-type tall fescue (Festuca arundinacea Schreb.) plants regenerated from protoplasts, Plant Cell Rep, 11, pp. 601-604, (1992)

[8]

Hartman C.L., Lee L., Day P.R., Turner N.E., Herbicide resistant turfgrass (Agrostis palustris Huds.) by biolistic transformation, Biotechnology, 12, pp. 919-923, (1994)

[9]

Horn M.E., Shillito R.D., Conger B.V., Harms C.T., Transgenic plants of Orchardgrass (Dactylis glomerata L.) from protoplasts, Plant Cell Rep, 7, pp. 469-1172, (1988)

[10]

Jauhar P.P., Cytogenetics of the Festuca-Lolium complex, Monogr Theor Appl Genet, 18, pp. 1-8, (1993)

← 1 2 3 →