Isolation and toxigenicity of Aspergillus fumigatus from moldy silage

被引：59

作者：

Valentina Melo dos Santos

Joe W. Dorner

Fátima Carreira

机构：

[1] Sector de Micologia, Lab. Regional de Veterinária, 9700-236 Angra do Heroismo, Acores

[2] National Peanut Research Laboratory, Dawson, GA

来源：

Mycopathologia | 2003年 / 156卷 / 2期

关键词：

Aspergillus fumigatus; Fumigaclavine; Fumitremorgen; Gliotoxin; Mycotoxins; Silage;

D O I：

10.1023/A:1022996911563

中图分类号：

学科分类号：

摘要：

Thirty-nine silage samples were collected from various silos on Terceira Island in the Azores. Samples were examined for the presence of total fungi, and isolates of Aspergillus fumigatus were analyzed for their ability to produce fumitremorgens B and C, fumigaclavines B and C, and gliotoxin. Thirty-four silage samples (87%) were contaminated with fungi, and A. fumigatus was isolated from 27 samples (69%). Samples that were taken from the surface of silos had significantly higher populations of both total fungi and A. fumigatus than did samples taken from the middle of silos. Analysis of 27 A. fumigatus isolates (one representing each positive sample) showed that 59.3% produced fumitremorgen B; 33.3% produced fumitremorgen C; 29.6% produced fumigaclavine B; 7.4% produced fumigaclavine C; and 11.1% produced gliotoxin. Fifty-two percent of the isolates produced multiple toxins, and 25.9% did not produce any of these toxins. Gliotoxin and fumigaclavine C were always produced in combination with other toxins. Because of the demonstrated potential of these A. fumigatus isolates to produce mycotoxins, it is important to properly construct and manage silos to prevent their contamination with A. fumigatus.

引用

页码：133 / 138

页数：5

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