Mapping the genome of Eimeria tenella: Use of a yeast artificial chromosome library as the basis for a physical map

被引：5

作者：

Hoogendoorn B. ^{[1
]}

机构：

[1] Institute for Animal Health, Compton

来源：

Parasitology Research | 1997年 / 84卷 / 1期

关键词：

Genetic Mapping; Size Fractionation; Reference Strain; Positive Clone; Nylon Membrane;

D O I：

10.1007/s004360050349

中图分类号：

学科分类号：

摘要：

Efforts to derive a physical map of the genome of Eimeria tenella are being made to complement genetic mapping and to assist in the production of an integrated map. A large insert library of DNA from the Houghton (H) reference strain of E. tenella has been constructed in yeast artificial chromosomes (YACs). The library contains a tenfold E. tenella genome equivalent consisting of 3177 clones arrayed in 96-well microtitre plates and gridded on nylon membranes at 1728 clones/86 cm2. Size fractionation of a random sample of 185 YACs revealed an average insert size of approximately 170 kb. Hybridisation of four E. tenella single-copy probes to the gridded colony filters produced around four or more positive clones. A probe representing the whole of the 1.2-Mb chromosome 2, excised from a preparative gel following pulsed-field gel electrophoresis, revealed between 80 and 100 positive clones. © Springer-Verlag 1998.

引用

页码：13 / 16

页数：3

共 3 条

[1]

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[2]

Den Dunnen J.T., Grootscholten P.M., Dauwerse J.G., Walker A.P., Monaco A.P., Butler R., Anand R., Coffey A.J., Bentley D.R., Steensma H.Y., Van Ommen G.J.B., Reconstruction of the 2.4 Mb human DMD-gene by homologous YAC recombination, Hum Mol Genet, 1, pp. 19-28, (1992)

[3]

Dietrich W.F., Copeland N.G., Gilbert D.J., Miller J.C., Jenkins N.A., Lander E.S., Mapping the mouse genome: Current status and future prospects, Proc Natl Acad Sci USA, 92, pp. 10849-10853, (1995)

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