Influencing the cytokine secretion of murine thymoma cells (EL-4) by ochratoxin A [Beeinflussung der Zytokinsekretion von Maus-Thymom-Zellen (EL-4) durch Ochratoxin A]

被引：2

作者：

Heller M. ^{[1
]}

Köhler H. ^{[1
]}

Rosner H. ^{[1
]}

Burkert B. ^{[1
]}

Rohrmann B. ^{[1
]}

Möller U. ^{[1
]}

Thierbach S. ^{[1
]}

Kielstein P. ^{[1
]}

Müller G. ^{[1
]}

机构：

[1] Bundesinstitut für Gesundheitlichen Verbraucherschutz und Veterinärmedizin, FB 4, D-07743 Jena

来源：

Mycotoxin Research | 2000年 / 16卷 / Suppl 2期

关键词：

D O I：

10.1007/BF02940036

中图分类号：

学科分类号：

摘要：

The murine thymoma cell line EL-4 was used as an in vitro T-cell model to assess the immunomodulating effect of pure Ochratoxin A (OTA) and an Aspergillus ochraceus raw toxin preparation. Cytokine production (IL-2, IL-4, IL-5, IL-6) and cell viability of PMA-stimulated EL-4 cells were investigated in the presence of OTA. The cytotoxic effect of the raw toxin could be observed at lower concentrations than pure OTA. The IC50 values were 3 μg/ml and 11 μg/ml, respectively. Increasing concentrations of both OTA preparations caused an inhibition of cytokine production, but the inhibition effect of the raw toxin was stronger than of pure OTA. This is supposed to be the effect of further up to now not characterized substances in the raw toxin. Differences in the susceptibility of the mechanisms of production and regulation of each cytokine are indicated by the different concentrations for inhibition effects. Both toxin preparations showed also stimulating effects on some cytokines (IL-2 and IL-6) while others (IL-4 and IL-5) were depressed at these OTA concentrations. This indicates the immunomodulating properties of the toxin.

引用

页码：194 / 198

页数：4

共 4 条

[1]

Harvey R.B., Elissalde M.H., Kubcna L.F., Weaver E.A., Cirrier D.E., Clement B.A., Immunotoxicity of ochtatoxin A to growing gilts, Amer. J. Vet. Res., 53, pp. 1966-1970, (1992)

[2]

Marin M.L., Murtha J., Dong W., Pesta J.J., Effects of mycotoxins on cytokine production and proliferation in EL-4 thymoma cells, J. Toxicol. Environment. Health, 48, pp. 379-396, (1996)

[3]

Muller G., Kielstein P., Rosner H., Berndt A., Heller M., Kohler H., Studies of the infuence of ochratoxin A on immune and defence reactions in weaners, Mycosis, 42, pp. 495-505, (1999)

[4]

Ziegler-Heitbrock H.W.L., Strobel M., Kieber D., Fingerle G., Schlunde T., Petersmann I., Ellwart J., Blumenstein M., Haas J.G., Differential expression of cytokines in human blood monocyte subpopulations, Blood, 79, pp. 503-511, (1992)

← 1 →