Metabolic alterations in genetically selected Drosophila strains with different longevities

被引：4

作者：

Buck S.A. ^{[1
]}

Arking R. ^{[1
]}

机构：

[1] Department of Biological Sciences, Wayne State University, Detroit

来源：

Journal of the American Aging Association | 2001年 / 24卷 / 4期

关键词：

NADP; Pentose; Antioxidant Defense System; Metabolic Alteration; Metabolic Pattern;

D O I：

10.1007/s11357-001-0016-2

中图分类号：

学科分类号：

摘要：

Sometime ago we obtained biomarker data suggesting that the earliest determining event in the expression of the extended longevity phenotype in our selected strains of Drosophila took place early in adult life at about 5-7 days of age. In a later series of experiments we documented that our La and Lb long lived strains underwent a specific up-regulation of the antioxidant defense system (ADS) genes and enzymes. This led to a reduction in oxidative damage and an extended longevity. In the current work, we assayed the activity of 17 metabolically important enzymes in 5-7 day old files of 13 strains variously selected for different longevities. We conclude that the two sets of replicated long-lived strains have an altered metabolic pattern (relative to normal-lived animals) which is consistent with an increased flux through the pentose shunt and an enhanced NADP+ reducing system to support the increased activity of the ADS enzymes. This result can be interpreted as a shift of energy expenditure from reproduction to somatic maintenance. We conclude that theories based on differential energy allocations appear to empirically explain, at least in part, the mechanisms underlying the transformation of a normal longevity phenotype to an extended longevity phenotype.

引用

页码：151 / 161

页数：10

共 29 条

[1]

Arking R., ed., (1998)

[2]

Kirkwood T.B.L., Immortality of the germ-live versus disposability of the soma, Evolution of Longevity in Animals: A Comparative Approach, pp. 209-218, (1987)

[3]

Partridge L., Barton N.H., Optimality, mutation and the evolution of ageing, Nature, 362, pp. 305-311, (1993)

[4]

Luckinbill L.S., Arking R., Clare M.J., Cirocco W.C., Buck S., Selection for delayed senescence in Drosophila melanogaster, Evolution, 38, pp. 996-1004, (1984)

[5]

Rose M.R., Laboratory evolution of postponed senescence in Drosophila melanogaster, Evolution, 38, pp. 1004-1010, (1984)

[6]

Arking R., Successful selection for increased longevity in Drosophila: Analysis of the survival data and presentation of a hypothesis on the genetic regulation of longevity, Exp. Gerontol., 22, pp. 199-220, (1987)

[7]

Dudas S.P., Arking R., A coordinate upregulation of antioxidant gene activities is associated with the delayed onset of senescence in a long-lived strain of Drosophila, J. Gerontol.: Biol. Sci., 50 A, (1995)

[8]

Arking R., Burde V., Graves K., Hari R., Feldman E., Zvei A., Soliman S., Saraiya A., Buck S., Vettraino J., Sathrasala K., Wehr N., Levine R.L., Forward and reverse selection for longevity in Drosophila is characterized by alteration of antioxidant gene expression and oxidative damage patterns, Exp. Gerontol., 35, pp. 167-185, (2000)

[9]

Arking R., Burde V., Graves K., Hari R., Feldman E., Zvei A., Soliman S., Saraiya A., Buck S., Vettraino J., Sathrasala K., Identical longevity phenotypes are characerized by different patterns of gene expression and oxidative damage, Exp. Gerontol., 35, pp. 33-373, (2000)

[10]

Arking R., Gene expression and regulation in the extended longevity phenotypes of Drosophila, Annals N.Y. Acad. Sci., 928, pp. 157-167, (2001)

← 1 2 3 →