Impact of different levels of cinnamyl alcohol dehydrogenase down-regulation on lignins of transgenic tobacco plants

被引：72

作者：

Yahiaoui N. ^{[1
]}

Marque C. ^{[1
]}

Myton K.E. ^{[1
]}

Negrel J. ^{[2
]}

Boudet A.M. ^{[1
]}

机构：

[1] UMR CNRS/UPS 5546, Ctr. de Biol. et Physiol. Veg., F-31062 Toulouse Cedex, 118, route de Narbonne

[2] Laboratoire de Phytoparasitologie, INRA-CNRS, INRA BV1540

来源：

Planta | 1997年 / 204卷 / 1期

关键词：

Antisense strategy; Cinnamyl aidehyde; Cinnamyl alcohol dehydrogenase; Lignin; Nicotiana; Transgenic tobacco;

D O I：

10.1007/s004250050224

中图分类号：

学科分类号：

摘要：

The effects of cinnamyl alcohol dehydrogenase (CAD, EC.1.1.1.195) down-regulation on lignin profiles of plants were analysed in four selected transgenic lines of tobacco (Nicotiana tabacum L. cv. Samsun) exhibiting different levels of CAD activity (8-56% of the control). A significant decrease in thioacidolysis yields (i.e. yield of β-0-4 linked monomers) and in the ratio of syringyl to guaiacyl monomers (S/G) was observed for three transgenic lines and the most drastic reduction (up to 50%) was correlated with the lowest level of CAD activity. Higher lignin extractability by mild alkali treatment was confirmed, and, in addition to a tenfold increase in C6-C1 aldehydes, coniferyl aldehyde was detected by high-performance liquid chromatography in the alkali extracts from the xylem of transgenic plants. In-situ polymerisation of cinnamyl aldehydes in stem sections of untransformed tobacco gave a xylem cell wall coloration strikingly similar to the reddish-brown coloration of the xylem of antisense CAD-down-regulated plants. Overall, these data provide new arguments for the involvement of polymerised cinnamyl aldehydes in the formation of the red-coloured xylem of CAD-downregulated plants.

引用

页码：8 / 15

页数：7

共 35 条

[1] Argillier O., Barrire Y., Lila M., Jeanneteau F., Gelinet K., Menanteau V., Genotypic variation in phenotic components of cell-walls in relation to the digestibility of maize stalks, Agronomie, 16, pp. 123-130, (1996)
[2] Bao W., O'Malley D.M., Whetten R., Sederoff R.R., A laccase associated with lignification in loblolly pine xylem, Science, 260, pp. 672-674, (1993)
[3] Baucher M., Chabbert B., Pilate G., Van Doorsselaere J., Tollier M.-T., Petit-Conil M., Cornu D., Monties B., Van Montagu M., Inze D., Jouanin L., Boerjan W., Red xylem and higher lignin extractability by down-regulating a cinnamyl alcohol dehydrogenase in poplar (Populus tremula × P. alba), Plant Physiol, 112, pp. 1479-1490, (1996)
[4] Bernard Vailhe M.A., Cornu A., Robert D., Maillot M., Besle J.M., Cell wall degradability of transgenic tobacco stems in relation to their chemical extraction and lignin quality, J Agric Food Chem, 44, pp. 1164-1169, (1996)
[5] Boudet A.M., Grima-Pettenati J., Lignin genetic engineering, Mol Breeding, 2, pp. 25-39, (1996)
[6] Boudet A.M., Lapierre C., Grima-Pettenati J., Biochemistry and molecular biology of lignification, New Phytol, 129, pp. 203-236, (1995)
[7] Bradford M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding, Anal Biochem, 72, pp. 248-254, (1976)
[8] Campbell M.M., Ellis B.E., Fungal elicitor-mediated responses in pine cell cultures: Cell wall bound phenolics, Phytochemistry, 31, pp. 737-742, (1992)
[9] Campbell M.M., Sederoff R.R., Variation in lignin content and composition, Plant Physiol, 110, pp. 3-13, (1996)
[10] Dean J.F.D., Eriksson K.-E.L., Biotechnological modification of lignin structure and composition in forest trees, Holzforschung, 46, pp. 135-147, (1992)

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