Minor groove RNA triplex in the crystal structure of a ribosomal frameshifting viral pseudoknot

被引：183

作者：

Su L. ^{[1
]}

Chen L. ^{[1
,2
]}

Egli M. ^{[3
]}

Berger J.M. ^{[1
,4
,5
]}

Rich A. ^{[1
]}

机构：

[1] Department of Biology, Massachusetts Inst. of Technology, Cambridge

[2] Laboratory for Structural Biology, Dept. of Chem. and Materials Science, University of Alabama, Huntsville

[3] Dept. Molec. Pharmacol./Biol. Chem., Drug Discovery Program, NW University Medical School, Chicago

[4] Whitehead Institute, Department of Biology, Massachusetts Inst. of Technology, Cambridge

[5] Dept. of Molecular and Cell Biology, University of California, Berkeley

来源：

Nature Structural Biology | 1999年 / 6卷 / 3期

基金：

美国国家卫生研究院; 美国国家科学基金会; 美国国家航空航天局;

关键词：

D O I：

10.1038/6722

中图分类号：

学科分类号：

摘要：

Many viruses regulate translation of polycistronic mRNA using a -1 ribosomal frameshift induced by an RNA pseudoknot. A pseudoknot has two stems that form a quasi-continuous helix and two connecting loops. A 1.6 Å crystal structure of the beet western yellow virus (BWYV) pseudoknot reveals rotation and a bend at the junction of the two stems. A loop base is inserted in the major groove of one stem with quadruple-base interactions. The second loop forms a new minor-groove triplex motif with the other stem, involving 2'-OH and triple-base interactions, as well as sodium ion coordination. Overall, the number of hydrogen bonds stabilizing the tertiary interactions exceeds the number involved in Watson-Crick base pairs. This structure will aid mechanistic analyses of ribosomal frameshifting.

引用

页码：285 / 292

页数：7

共 15 条

[1] Gesteland R.F., Atkins J.F., Recoding: Dynamic reprogramming of translation, Annu. Rev. Biochem., 65, pp. 741-768, (1996)
[2] Farabaugh P.J., Programmed translational frameshifting, Microbiol. Rev., 60, pp. 103-134, (1996)
[3] Jacks T., Madhani H.D., Masiarz F.R., Varmus H.E., Signals for ribosomal frameshifting in the Rous sarcoma virus gag-pol region, Cell, 55, pp. 447-458, (1988)
[4] Chamorro M., Parkin N., Varmus H.E., An RNA pseudoknot and an optimal heptameric shift site are required for highly efficient ribosomal frameshifting on a retroviral messenger RNA, Proc. Natl. Acad. Sci. USA, 89, pp. 713-717, (1992)
[5] Ten Dam E., Brierley I., Inglis S., Pleij C., Identification and analysis of the pseudoknot-containing gag-pro ribosomal frameshift signal of simian retrovirus-1, Nucleic Acids Res., 22, pp. 2304-2310, (1994)
[6] Brierley I., Rolley N.J., Jenner A.J., Inglis S.C., Mutational analysis of the RNA pseudoknot component of a coronavirus ribosomal frameshifting signal, J. Mol. Biol., 220, pp. 889-902, (1991)
[7] Tzeng T.-H., Tu C.-L., Bruenn J.A., Ribosomal frameshifting requires a pseudoknot in the Saccharomyces cerevisiae double-stranded RNA virus, J. Virol., 66, pp. 999-1006, (1992)
[8] Miller W.A., Dinesh-Kumar S.P., Paul C.P., Luteovirus gene expression, Crit. Rev. Plant Sci., 14, pp. 179-211, (1995)
[9] Somogyi P., Jenner A.J., Brierley I., Inglis S.C., Ribosomal pausing during translation of an RNA pseudoknot, Mol. Cell. Biol., 13, pp. 6931-6940, (1993)
[10] Pleij C.W.A., Rietveld K., Bosch L., A new principle of RNA folding on pseudoknotting, Nucleic Acids Res., 13, pp. 1717-1731, (1985)

← 1 2 →