A Method for Isolating Total RNA from Pear Leaves

被引：52

作者：

Malnoy M. ^{[1
]}

Reynoird J.P. ^{[1
,2
]}

Mourgues F. ^{[1
,3
]}

Chevreau E. ^{[1
]}

Simoneau P. ^{[4
]}

机构：

[1] INRA U. d'Amelioration Especes F., 49071 Beaucouzé Cedex

[2] Dept. of Plant Pathology, Cornell University, Geneva

[3] Fruit Tree Research Station ISF, Forli Section, 47100 Forli

[4] UMR 077 Pathol. Veg., Faculté des Sciences, 49045 Angers Cedex

来源：

Plant Molecular Biology Reporter | 2001年 / 19卷 / 1期

关键词：

Pear; Polysaccharide; RNA contamination; RNA extraction;

D O I：

10.1007/BF02824081

中图分类号：

学科分类号：

摘要：

Isolation of high quality RNA from Rosaceae species is particularly difficult. These plants contain considerable amounts of plant polyphenolic compounds and polysaccharides that copurify with RNA, often rendering it unsuitable for either cDNA synthesis and/or hybridization in northern analyses. We describe a method for RNA isolation from pear leaves that is modified from that of Manning (1990). The procedure includes i) an extraction with phenol and PVPP, to remove proteins and polyphenols ii) two purifications by LiCl, with a 2-butoxyethanol treatment between the LiCl steps. The method results in high quality RNA suitable for RT-PCR and northern blot experiments.

引用

页码：69a / 69f

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