CHARACTERIZING CHEMICAL-SYSTEMS WITH ONLINE COMPUTERS AND GRAPHICS - ALKALINE-PHOSPHATASE CATALYZED REACTION

被引：19

作者：

FRAZER, JW ^{[1
]}

RIGDON, LP ^{[1
]}

BRAND, HR ^{[1
]}

POMERNACKI, CL ^{[1
]}

BRUBAKER, TA ^{[1
]}

机构：

[1] COLORADO STATE UNIV,DEPT ELECT ENGN,FT COLLINS,CO 80523

来源：

ANALYTICAL CHEMISTRY | 1979年 / 51卷 / 11期

关键词：

D O I：

10.1021/ac50047a036

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Computer automation of experimental apparatus can aid in the global characterization of complex systems by rapidly providing large quantities of accurate data. The combination of fast data collection and manipulation provides a means for understanding complex chemical systems that first appear to be medium number systems. We have applied graphical techniques to the study of the alkaline phosphatase-catalyzed hydrolysis of p-nitrophenylphosphate. Three dimensional Box-type surfaces gave a quick visual summary of the reaction. Model response surfaces were also generated and compared with experimental surfaces to evaluate the model and analytical techniques. Residual-surface graphic techniques that compare experimental and model response surfaces aid in the identification of modeling or experimentation errors. Reaction rate surfaces, which are three-dimensional plots at constant reaction rate, are a new type of graphical representation of use in presenting complex nonlinear systems. Traditional plots and replots are also generated on-line. © 1979, American Chemical Society. All rights reserved.

引用

页码：1747 / 1754

页数：8

共 10 条

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[5] DIXON M, 1964, ENZYMES, pCH4
[6] FRAZER JW, 1979, ANAL CHEM, V51, P1739, DOI 10.1021/ac50047a035
[7] FRAZER JW, UNPUBLISHED
[8] Segel LH, 1975, ENZYME KINETICS
[9] Weinberg G., 1975, INTRO GENERAL SYSTEM
[10] ZEFFREN E, 1973, STUDY ENZYME MECHANI, pCH5

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