NEUROSPORA-CRASSA A MATING-TYPE REGION

被引：183

作者：

GLASS, NL ^{[1
]}

GROTELUESCHEN, J ^{[1
]}

METZENBERG, RL ^{[1
]}

机构：

[1] UNIV WISCONSIN,DEPT PHYSIOL CHEM,MADISON,WI 53706

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 13期

关键词：

filamentous fungus; sexual reproduction; vegetative incompatibility;

D O I：

10.1073/pnas.87.13.4912

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The mating-type locus of the haploid filamentous fungus Neurospora crassa is a regulatory region that controls entry into the sexual cycle and prevents formation of mixed mating-type heterokaryons in the vegetative phase. The locus consists of alternative sequences called A and a. The A mating-type DNA sequence of Neurospora crassa is composed of a region of 5301 base pairs that has little similarity to the sequence present at the mating-type locus in an a mating-type strain. However, the sequences flanking the mating-type locus in the A haploid and a haploid genome are essentially identical. The region of the A mating-type sequence required for expression of the heterokaryon incompatibility and sexual functions has been localized to a single open reading frame (ORF) encoding a polypeptide of 288 amino acids. Sequence analysis of sterile, heterokaryon-compatible mutants reveals frameshift mutations in this same ORF. The putative 288-amino acid product has a region of similarity to the MATα1 polypeptide of Saccharomyces cerevisiae.

引用

页码：4912 / 4916

页数：5

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