NEUROSPORA-CRASSA A MATING-TYPE REGION

被引:183
作者
GLASS, NL [1 ]
GROTELUESCHEN, J [1 ]
METZENBERG, RL [1 ]
机构
[1] UNIV WISCONSIN,DEPT PHYSIOL CHEM,MADISON,WI 53706
关键词
filamentous fungus; sexual reproduction; vegetative incompatibility;
D O I
10.1073/pnas.87.13.4912
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The mating-type locus of the haploid filamentous fungus Neurospora crassa is a regulatory region that controls entry into the sexual cycle and prevents formation of mixed mating-type heterokaryons in the vegetative phase. The locus consists of alternative sequences called A and a. The A mating-type DNA sequence of Neurospora crassa is composed of a region of 5301 base pairs that has little similarity to the sequence present at the mating-type locus in an a mating-type strain. However, the sequences flanking the mating-type locus in the A haploid and a haploid genome are essentially identical. The region of the A mating-type sequence required for expression of the heterokaryon incompatibility and sexual functions has been localized to a single open reading frame (ORF) encoding a polypeptide of 288 amino acids. Sequence analysis of sterile, heterokaryon-compatible mutants reveals frameshift mutations in this same ORF. The putative 288-amino acid product has a region of similarity to the MATα1 polypeptide of Saccharomyces cerevisiae.
引用
收藏
页码:4912 / 4916
页数:5
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