NUCLEAR DNA CONTENT OF VITIS SPECIES, CULTIVARS, AND OTHER GENERA OF THE VITACEAE

被引：72

作者：

LODHI, MA ^{[1
]}

REISCH, BI ^{[1
]}

机构：

[1] CORNELL UNIV, DEPT HORT SCI, NEW YORK STATE AGR EXPT STN, GENEVA, NY 14456 USA

来源：

THEORETICAL AND APPLIED GENETICS | 1995年 / 90卷 / 01期

关键词：

AMPELOPSIS; FLOW CYTOMETRY; GRAPEVINES; PARTHENOCISSUS;

D O I：

10.1007/BF00220990

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The nuclear DNA content was analyzed in Vitis species, hybrid cultivars, and genera of the Vitaceae using flow cytometry. Significant variation was found among Vitis species, hybrids, and other genera of the Vitaceae (Ampelopsis and Parthenocissus). DNA content was estimated to range from 0.98 to 1.05 pg/2C within V. labrusca (ns) and 0.86 to 1.00 pg/2C within V. vinifera (ns). Genotypes from Vitis and Parthenocissus were similar in nuclear DNA content (approximately 1.00 pg/2C) whereas they differed significantly from Ampelopsis (1.39 pg/2C). No correlation between DNA content and the center of origin of genotypes of the Vitaceae was noted. Based on the present study, the Vitis genome size is 475 Mbp, 96% of which is non-coding. Knowledge of DNA content is useful in order to understand the complexity of the Vitis genome and to establish a relationship between the genetic and physical map for map-based cloning.

引用

页码：11 / 16

页数：6

共 65 条

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