STUDIES ON METHIONYL-TRNA SYNTHETASE .2. EFFECTS OF DIVALENT AND MONOVALENT CATIONS ON METHIONYL-TRNA SYNTHETASE FROM ESCHERICHIA COLI

被引：15

作者：

SVENSSON, I

机构：

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1967年 / 146卷 / 01期

关键词：

D O I：

10.1016/0005-2744(67)90091-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of divalent and monovalent cations on the activity of methionyl-tRNA synthetase (L-methionine: tRNA ligase (AMP), EC 6.1.1.10) from E.coli was studied, and a comparison had been made with the corresponding enzyme from yeast. Of the monovalent cations, only NH4+ stimulates the rate of methionyl-tRNA formation. This effect is found with both homologous and heterologous tRNA. The magnitude of the stimulation varies with pH. The results explain a phenomenon previously interpreted in terms of a "regenerating" enzyme in amino acid activation.

引用

页码：253 / +

页数：1

共 9 条

[1] EVIDENCE FOR A NEW ENZYME CONNECTED WITH PROTEIN SYNTHESIS [J].