SEPARATION OF O-PHTHALALDEHYDE MERCAPTOETHANOL DERIVATIVES OF AMINO-ACIDS FROM BLOOD-PLASMA ON REVERSED-PHASE NOVA-PAK C18 CARTRIDGES

被引：8

作者：

SCHMIDT, J ^{[1
]}

MCCLAIN, CJ ^{[1
]}

机构：

[1] VET ADM MED CTR,LEXINGTON,KY 40511

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 568卷 / 01期

关键词：

D O I：

10.1016/0378-4347(91)80354-F

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A separation of 25 o-phthalaldehyde-mercaptoethanol derivatives of primary amino acids in plasma prepared from human blood has been developed for Waters 10 cm x 0.8 cm I.D., 4-mu-m Nova-Pak C18 Radial-Pak catridges. A binary gradient system with solvent-switching capability for the A pump is required. Computer methodologies have been utilized to develop mobile phase mixtures of phosphate buffer (pH 6.9), water, methanol and tetrahydrofuran. Advantages of the method include simple sample preparation, fast turnover time (67 min including the pre-column Autotag derivatization procedure) and exceptional column durability (several hundred analyses).

引用

页码：207 / 211

页数：5

共 9 条

[1] FLUORESCENCE DETECTION OF CYSTINE BY ORTHO-PHTHALALDEHYDE DERIVATIZATION AND ITS SEPARATION USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].