CRYSTALLIZATION OF THE SERYL-TRANSFER RNA SYNTHETASE-TRANSFER RNASER COMPLEX FROM THERMUS-THERMOPHILUS

被引：10

作者：

YAREMCHUK, AD

TUKALO, MA

KRIKLIVIY, IA

MELNIK, VN

BERTHETCOLOMINAS, C

CUSACK, S

LEBERMAN, R

机构：

[1] EUROPEAN MOLEC BIOL LAB, GRENOBLE OUTSTN 156X, F-38042 GRENOBLE, FRANCE

[2] ACAD SCI UKSSR, INST MOLEC BIOL & GENET, KIEV 252627, UKRAINE, USSR

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 224卷 / 02期

关键词：

AMINOACYL-TRANSFER RNA SYNTHETASE; TRANSFER RNA; CRYSTALLIZATION; THERMUS-THERMOPHILUS;

D O I：

10.1016/0022-2836(92)91014-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complex between seryl-tRNA synthetase and its cognate tRNA from the extreme thermophile Thermus thermophilus has been crystallized from ammonium sulphate solutions. Two different tetragonal crystal forms have been characterized, both diffracting to about 6 Å using synchrotron radiation. One form grows as large bipyramids and has cell dimensions a = b = 127 A ̊, c = 467 A ̊, and the second form occurs as long, thin square prisms with cell dimensions a = b = 101 A ̊, c = 471 A ̊. Analysis of washed and dissolved crystals demonstrates the presence of both protein and tRNA. © 1992.

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页码：519 / 522

页数：4

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