DISRUPTION OF U8 NUCLEOLAR SNRNA INHIBITS 5.8S AND 28S RIBOSOMAL-RNA PROCESSING IN THE XENOPUS-OOCYTE

被引：220

作者：

PECULIS, BA

STEITZ, JA

机构：

[1] Department of Molecular Biophysics, Biochemistry Howard Hughes Medical Institute Yale University School of Medicine New Haven

来源：

CELL | 1993年 / 73卷 / 06期

关键词：

D O I：

10.1016/0092-8674(93)90651-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nucleoli of vertebrate cells contain several snRNPs, of which only one, U3, has been assigned a role in rRNA processing. We present the primary sequence of Xenopus U8, a fibrillarin-associated nucleolar snRNA, and examine its expression through oocyte development. Antisense deoxyoligonucleotides were microinjected into Xenopus oocytes to deplete the endogenous pool of U8 RNA. Analysis of the mature rRNAs and rRNA intermediates that accumulate in the U8-depleted oocytes indicate that the U8 snRNP is essential for correct maturation of the 5.8S and 28S rRNAs at both their 5' and 3' ends. U8 is therefore a nucleolar snRNA implicated in a nucleolytic rRNA processing step other than 18S maturation. Evidence for a long-lived 5.8S rRNA intermediate (12S) in Xenopus is also presented.

引用

页码：1233 / 1245

页数：13

共 56 条

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