STRUCTURE OF PHOSPHATIDYLCHOLINES OF LUNG SURFACTANT AT BIRTH IN NORMAL FULL TERM INFANTS

被引：8

作者：

GIUDICELLI, Y

PECQUERY, R

LACASA, M

BOSCAMERIC, M

NORDMANN, R

机构：

[1] FAC MED PARIS QUEST, BIOCHIM SERV, PARIS, FRANCE

[2] CTR HOSP INTERCOMMUNAL POISSY, BIOCHIM LAB, 78303 PARIS, FRANCE

来源：

CLINICA CHIMICA ACTA | 1976年 / 71卷 / 03期

关键词：

D O I：

10.1016/0009-8981(76)90096-6

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The procedure, using tracheal aspirates as lung secretions, combined a cold-acetone precipitation and a 2-dimensional TLC of the lipid extract. Different species of phosphatidylcholines were isolated and found to account together for over 60% of the total phospholipids in tracheal aspirates. Analysis of the fatty acids esterifying the .alpha.- and .beta.-carbon of these different phosphatidylcholines showed palmitic acid as the major component with little myristic acid. The fatty acid analysis revealed furthermore that the major phosphatidylcholine fraction was almost exclusively .alpha.,.beta.-dipalmitoylphosphatidylcholine. The procedure described provides a useful and simple method for the extraction, isolation and characterization of the functional components of lung surfactant in living human newborns.

引用

页码：445 / 459

页数：15

共 32 条

[1] CONVERSION OF LYSOLECITHIN TO LECITHIN IN SUPERNATANT FRACTIONS OF VARIOUS RAT TISSUES - DISTRIBUTION OF SO-CALLED MARINETTIS ENZYME [J].