ADSORPTION OF PEPSIN BY ALUMINUM HYDROXIDE .1. ADSORPTION MECHANISM

被引:44
作者
SEPELYAK, RJ
FELDKAMP, JR
MOODY, TE
WHITE, JL
HEM, SL
机构
[1] PURDUE UNIV, DEPT IND PHARM, W LAFAYETTE, IN 47907 USA
[2] PURDUE UNIV, DEPT PHYS PHARM, W LAFAYETTE, IN 47907 USA
[3] PURDUE UNIV, DEPT AGRON, W LAFAYETTE, IN 47907 USA
关键词
D O I
10.1002/jps.2600731104
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Adsorption of pepsin by gibbsite and boehnite, non-acid-reactive forms of Al(OH)3 [widely used in peptic ulcer therapy], was observed and related to the surface area of the adsorbent. Adsorption was pH dependent, with maximum adsorption occurring between pH 2.7-3.3 for gibbsite and pH 2.7-4.3 for boehmite. Electrostatic attraction was an important adsorption mechanism at the pH conditions encountered in the GI [gastrointestinal] tract; the isoelectric point of pepsin was .apprx. 1, giving it a negative charge, and the point of zero charge for the adsorbents was > 9, giving them a positive charge. The pH-adsorption profile cannot be fully explained by electrostatic considerations. Desorption studies indicate the importance of specific adsorption because pepsin was not desorbed by washing with acidified water, but was partly desorbed by exchange with phosphate. The IR spectrum of adsorbed pepsin also suggested that specific adsorption of pepsin occurred through anionic ligand exchange involving carboxylate groups of pepsin and surface Al ions.
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页码:1514 / 1517
页数:4
相关论文
共 47 条
[1]  
ANDERSON W, 1972, J PHARM PHARMACOL, V24, pP166
[3]  
BEGEMANN F, 1981, SCAND J GASTROENTERO, V16, P191
[4]  
BERSTAD A, 1979, SCAND J GASTROENTERO, V14, P121
[5]  
BOWDEN JW, 1973, NATURE-PHYS SCI, V245, P81, DOI 10.1038/physci245081a0
[6]  
BOYD L, 1942, REV GASTROENTEROL, V9, P20
[7]   EFFECT OF ADSORBED CARBONATE ON SURFACE-CHARGE CHARACTERISTICS AND PHYSICAL-PROPERTIES OF ALUMINUM HYDROXIDE GEL [J].
FELDKAMP, JR ;
SHAH, DN ;
MEYER, SL ;
WHITE, JL ;
HEM, SL .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1981, 70 (06) :638-640
[8]  
FRASER RDB, 1970, PHYSICAL PRINCIPLES, P265
[9]  
HARTSUCK JA, 1972, J BIOL CHEM, V247, P2575
[10]   SPECIFIC ADSORPTION OF ANIONS [J].
HINGSTON, FJ ;
ATKINSON, RJ ;
POSNER, AM ;
QUIRK, JP .
NATURE, 1967, 215 (5109) :1459-+