PHOSPHORIBOSYLPYROPHOSPHATE SYNTHETASE IN HUMAN ERYTHROCYTES - ASSAY AND KINETIC-STUDIES USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：9

作者：

SAKUMA, R

NISHINA, T

YAMANAKA, H

KAMATANI, N

NISHIOKA, K

MAEDA, M

TSUJI, A

机构：

[1] OKINAKA MEM INST MED RES MINATO KU, TOKYO, JAPAN

[2] TOKYO WOMENS MED COLL, INST RHEUMATOL, SHINJUKU KU, TOKYO 162, JAPAN

[3] SHOWA UNIV, SCH PHARMACEUT SCI, SHINAGAWA KU, TOKYO 142, JAPAN

来源：

CLINICA CHIMICA ACTA | 1991年 / 203卷 / 2-3期

关键词：

HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; PHOSPHORIBOSYLPYROPHOSPHATE SYNTHETASE; GOUT; PYRIMIDINE 5'-NUCLEOTIDASE DEFICIENCY;

D O I：

10.1016/0009-8981(91)90286-L

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A method using high-performance liquid chromatography (HPLC) for determination of phosphoribosylpyrophosphate (PRPP) synthetase activity in human erythrocytes has been developed and PRPP synthetase activity on purine and pyrimidine metabolic disorders has been studied. Kinetic properties of erythrocyte PRPP synthetase of patients with gout and of a patient with pyrimidine 5'-nucleotidase deficiency were compared with those of healthy subjects. The mean of PRPP synthetase activity of gouty patients was a little higher (P < 0.01) than that of healthy subjects. The response of the enzyme for ATP of gouty patients was different from that of healthy subjects. The shapes of activation curve of the enzyme for inorganic phosphate were hyperbolic in gouty patients and in a patient with pyrimidine 5'-nucleotidase deficiency.

引用

页码：143 / 152

页数：10

共 21 条

[1] GOUT WITH PURINE OVERPRODUCTION DUE TO INCREASED PHOSPHORIBOSYLPYROPHOSPHATE SYNTHETASE-ACTIVITY [J].