A NOVEL UBIQUINONE REDUCTASE-ACTIVITY IN RAT CYTOSOL

被引：32

作者：

TAKAHASHI, T

SHITASHIGE, M

OKAMOTO, T

KISHI, T

GOSHIMA, K

机构：

[1] KOBE GAKUIN UNIV,FAC PHARMACEUT SCI,NISHI KU,KOBE 65121,JAPAN

[2] KOBE GAKUIN UNIV,FAC HUMANITIES & SCI,NISHI KU,KOBE 65121,JAPAN

来源：

FEBS LETTERS | 1992年 / 314卷 / 03期

关键词：

NAD(P)H; DT-DIAPHORASE; CYTOSOL (RAT LIVER); UBIQUINONE REDUCTASE; SUBCELLULAR ORGANELLE;

D O I：

10.1016/0014-5793(92)81499-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ubiquinone (UQ) reductase activity which reduces UQ to ubiquinol (UQH2) in rat tissues was roughly proportional to the UQH2/total UQ ratio in respective tissues. The highest activity was found in the liver, showing the highest UQH2/total UQ ratio. A greater part of liver UQ reductase activity was located in the cytosol. Within a week, the liver UQ reductase activity decreased by 80% even at -20-degrees-C. The DT-diaphorase activity was stable. UQ reductase required NADPH as the hydrogen donor and was not inhibited by a less than 1 muM concentration of dicoumarol. There was no stimulation of UQ reductase in the presence of bovine serum albumin nor in Triton X-100. Yet, both stimulated DT-diaphorase. As a result, UQ reductase appeared to be a novel NADPH-UQ oxidoreductase and responsible for the UQ redox state in liver.

引用

页码：331 / 334

页数：4

共 20 条

[1] DISTRIBUTION AND REDOX STATE OF UBIQUINONES IN RAT AND HUMAN TISSUES [J].