HOMOLOGY BETWEEN PROTEINS CONTROLLING STREPTOMYCES-FRADIAE TYLOSIN RESISTANCE AND ATP-BINDING TRANSPORT

被引:75
作者
ROSTECK, PR
REYNOLDS, PA
HERSHBERGER, CL
机构
[1] Cell Culture Research and Development, Lilly Research Laboratories, Eli Lilly and Co., Indianapolis
关键词
RECOMBINANT DNA; MACROLIDE RESISTANCE; NUCLEOTIDE SEQUENCE; ANTIBIOTIC EXPORT; GENE DUPLICATION; TLRC;
D O I
10.1016/0378-1119(91)90533-H
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A tylosin(Ty)-producing strain of Streptomyces fradiae contains at least three genes, tlrA, tlrB, tlrC, specifying resistance to Ty (Ty(R)). The complete nucleotide sequence of the Ty(R)-encoding gene, tlrC, and the transcription start point of the gene were determined. The sequence contains an open reading frame coding for a protein of 548 amino acids (aa) with an M(r) of 59 129. The TlrC protein was identified by expression of the cloned gene by in vitro coupled transcription and translation in cell-free extracts derived from Streptomyces lividans. The N- and C-terminal halves of TlrC share extensive homology, suggesting that the protein evolved through tandem gene duplication. Each half of the deduced TlrC aa sequence also shows significant homology to numerous eukaryotic and prokaryotic membrane-associated, active-transport protein subunits. The homologous proteins include examples from the systems responsible for efflux of cytotoxic drugs from multidrug-resistant human cells and for export of hemolysin from Escherichia coli. The greatest similarity to TlrC is in regions containing the ATP-binding sites found in these proteins. These results suggest a role for the tlrC gene product as part of a multiple component, ATP-dependent transport system for the active excretion of Ty from the producing organism.
引用
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页码:27 / 32
页数:6
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