CIS ACTING RNA SEQUENCES CONTROL THE GAG POL TRANSLATION READTHROUGH IN MURINE LEUKEMIA-VIRUS

被引：35

作者：

HONIGMAN, A ^{[1
]}

WOLF, D ^{[1
]}

YAISH, S ^{[1
]}

FALK, H ^{[1
]}

PANET, A ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM, HADASSAH MED SCH, DEPT INTERNAL MED A, IL-91010 JERUSALEM, ISRAEL

来源：

VIROLOGY | 1991年 / 183卷 / 01期

关键词：

D O I：

10.1016/0042-6822(91)90144-Z

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The pol gene of the Moloney murine leukemia virus (M-MuLV) is expressed as a Gag-Pol fusion protein through an in-frame suppression of the UAG termination codon located between the two genes. The role of nucleotide context in suppression was investigated, in a rabbit reticulocyte lysate translation system, using site-directed mutagenesis. The results indicate that the translational readthrough is mediated by at least 50 bases long RNA sequence located 3′ to the gag UAG termination codon. Within this sequence a short purine-rich sequence adjacent to the amber codon, highly conserved among different retroviruses, appears essential for M-MuLV suppression. Two alternative putative stem and loop like RNA structures can be drawn at the gag-pol junction, one abutting the gag UAG codon, and the second downstream to it. None of these structures appears to be important to the suppression process. © 1991.

引用

页码：313 / 319

页数：7

共 34 条

[1] CONTEXT EFFECTS - TRANSLATION OF UAG CODON BY SUPPRESSOR TRANSFER-RNA IS AFFECTED BY THE SEQUENCE FOLLOWING UAG IN THE MESSAGE [J].

BOSSI, L .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 164 (01) :73-87

[2] CHARACTERIZATION OF AN EFFICIENT CORONAVIRUS RIBOSOMAL FRAMESHIFTING SIGNAL - REQUIREMENT FOR AN RNA PSEUDOKNOT [J].

BRIERLEY, I ;

DIGARD, P ;

INGLIS, SC .

CELL, 1989, 57 (04) :537-547

[3] UGA SUPPRESSION BY NORMAL TRANSFER RNATRP IN ESCHERICHIA-COLI - CODON CONTEXT EFFECTS [J].

ENGELBERGKULKA, H .

NUCLEIC ACIDS RESEARCH, 1981, 9 (04) :983-991

[4] EXPRESSION OF THE GAG-POL FUSION PROTEIN OF MOLONEY MURINE LEUKEMIA-VIRUS WITHOUT GAG PROTEIN DOES NOT INDUCE VIRION FORMATION OR PROTEOLYTIC PROCESSING [J].

FELSENSTEIN, KM ;

GOFF, SP .

JOURNAL OF VIROLOGY, 1988, 62 (06) :2179-2182

[5] SUPPRESSION OF UAA AND UGA TERMINATION CODONS IN MUTANT MURINE LEUKEMIA VIRUSES [J].

FENG, YX ;

LEVIN, JG ;

HATFIELD, DL ;

SCHAEFER, TS ;

GORELICK, RJ ;

REIN, A .

JOURNAL OF VIROLOGY, 1989, 63 (06) :2870-2873

[6] TRANSLATIONAL READTHROUGH OF THE MURINE LEUKEMIA-VIRUS GAG-GENE AMBER CODON DOES NOT REQUIRE VIRUS-INDUCED ALTERATION OF TRANSFER-RNA [J].

FENG, YX ;

HATFIELD, DL ;

REIN, A ;

LEVIN, JG .

JOURNAL OF VIROLOGY, 1989, 63 (05) :2405-2410

[7] GLUTAMINE STARVATION OF MURINE LEUKEMIA VIRUS-INFECTED CELLS INHIBITS THE READTHROUGH OF THE GAG-POL GENES AND PROTEOLYTIC PROCESSING OF THE GAG POLYPROTEIN [J].

GLOGER, I ;

PANET, A .

JOURNAL OF GENERAL VIROLOGY, 1986, 67 :2207-2213

[8] NUCLEOTIDE-SEQUENCE OF AKV MURINE LEUKEMIA-VIRUS [J].

HERR, W .

JOURNAL OF VIROLOGY, 1984, 49 (02) :471-478

[9] EXPRESSION OF THE ROUS-SARCOMA VIRUS POL GENE BY RIBOSOMAL FRAMESHIFTING [J].

JACKS, T ;

VARMUS, HE .

SCIENCE, 1985, 230 (4731) :1237-1242

[10] CHARACTERIZATION OF RIBOSOMAL FRAMESHIFTING IN HIV-1 GAG-POL EXPRESSION [J].

JACKS, T ;

POWER, MD ;

MASIARZ, FR ;

LUCIW, PA ;

BARR, PJ ;

VARMUS, HE .

NATURE, 1988, 331 (6153) :280-283

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