CULTURED MYOMETRIAL CELLS ESTABLISH COMMUNICATING GAP-JUNCTIONS

被引：20

作者：

CARUSO, RL

JUBERG, DR

CALDWELL, V

CORCOS, IA

机构：

[1] Toxicology Program, Department of Environmental and Industrial Health, The University of Michigan School of Public Health, Ann Arbor, MI 48109-2029

来源：

CELL BIOLOGY INTERNATIONAL REPORTS | 1990年 / 14卷 / 10期

关键词：

cell communication; Gap junctions; intercellular junctions; myometrium; rat; uterus;

D O I：

10.1016/0309-1651(90)91160-6

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Myometrial cells were isolated and cultured from term rat uterus. The myometrial origin of the cultures was verified by antibody staining of cellular desmin and α-smooth muscle actin. The presence of functional gap junctions was indicated by transfer of radiolabeled nucleotide and microinjected Lucifer yellow dye. The cultured cells expressed mRNA recognized by a connexin43 gap junction cDNA probe. To our knowledge, this is the first report that isolated myometrial cells form gap junctions in culture. © 1990.

引用

页码：905 / 916

页数：12

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