MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF CDNA CODING FOR RAT-LIVER HEMOPROTEIN H-450

被引：22

作者：

ISHIHARA, S

MOROHASHI, KI

SADANO, H

KAWABATA, SI

GOTOH, O

OMURA, T

机构：

[1] KYUSHU UNIV,GRAD SCH MED SCI,DEPT MOLEC BIOL,HIGASHI KU,FUKUOKA 812,JAPAN

[2] KYUSHU UNIV,MED INST BIOREGULAT,HIGASHI KU,FUKUOKA 812,JAPAN

[3] SAITAMA CANC CTR,RES INST,DEPT BIOCHEM,INA,SAITAMA 362,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1990年 / 108卷 / 06期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a123310

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

cDNA clones coding for hemoprotein H-450 were isolated from a rat liver cDNA library using anti-H-450 antibody. The molecular weight calculated from the deduced amino acid sequence comprising 547 amino acid residues was 60,085. The N-terminal sequence and a partial internal amino acid sequence of purified H-450, which were determined chemically, were both found in the amino acid sequence of H-450 deduced from the nucleotide sequence. H-450 mRNA is expressed in liver, kidney, and brain. A homology search of amino acid sequences indicated that H-450 shows no homology with cytochrome P-450, but shows significant homology with bacterial O-acetylserine (thiol)-lyases. However, H-450 has no O-acetylserine (thiol)-lyase activity. © 1990 Copyright, 1990 by the Journal of Biochemistry.

引用

页码：899 / 902

页数：4

共 27 条

[1]

BECKER MA, 1969, J BIOL CHEM, V244, P2418

[2]

Binkley F, 1942, J BIOL CHEM, V144, P507

[3] DNA-SEQUENCES OF THE CYSK REGIONS OF SALMONELLA-TYPHIMURIUM AND ESCHERICHIA-COLI AND LINKAGE OF THE CYSK REGIONS TO PTSH [J].