ENHANCEMENT OF SUBTILISIN-CATALYZED INTERESTERIFICATION IN ORGANIC-SOLVENTS BY ULTRASOUND IRRADIATION

被引：42

作者：

VULFSON, EN

SARNEY, DB

LAW, BA

机构：

[1] Department of Biotechnology and Enzymology, AFRC Institute of Food Research, Shinfield, Reading

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1991年 / 13卷 / 02期

关键词：

SUBTILISIN; ORGANIC SOLVENTS; ULTRASOUND IRRADIATION;

D O I：

10.1016/0141-0229(91)90166-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of ultrasound irradiation on subtilisin-catalysed interesterification of N-acetyl-phenylalanine ethyl ester in various alcohols has been studied. The pretreatment of subtilisin suspension by ultrasound led to a substantial increase of enzyme activity. The effect appeared to be dependent on the amplitude of sonication and the water content of the reaction medium; it was more pronounced in long-chain alcohols. An enhancement of reaction rate was observed with continuously sonicated preparations of the enzyme compared to those pretreated with ultrasound. Subtilisin was found to be much more resistant to inactivation by ultrasound irradiation in organic solvents than in water.

引用

页码：123 / 126

页数：4

共 12 条

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