PRODUCTION AND CHARACTERIZATION OF AN ANTIMELANOMA MONOCLONAL-ANTIBODY KBA.62 USING A NEW MELANOMA CELL-LINE REACTIVE ON PARAFFIN WAX EMBEDDED SECTIONS

被引:22
作者
COHENKNAFO, E
ALSAATI, T
AZIZA, J
RALFKIAER, E
SELVES, J
GORGUET, B
DELSOL, G
机构
[1] CHU PURPAN, DEPT PATHOL, ANAT PATHOL LAB, F-31059 TOULOUSE, FRANCE
[2] CHU PURPAN, CIGH, CNRS, F-31059 TOULOUSE, FRANCE
[3] UNIV COPENHAGEN, HERLEV, DENMARK
[4] HERLEV HOSP, DEPT PATHOL, HERLEV, DENMARK
关键词
KBA.62; MELANOMA; PARAFFIN WAX SECTIONS;
D O I
10.1136/jcp.48.9.826
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Aims-To generate new monoclonal antibodies directed against melanoma associated antigens using a new melanoma cell line, KAL. Methods-The melanoma cell line was established in culture from a lymph node metastasis of malignant melanoma. Normal Balb/c mice were immunised with KAL cells. Splenocytes were used for fusion experiments using standard techniques. Hybridoma supernatants were tested for antibody binding activity using an indirect immunoperoxidase method on frozen sections from KAL tumour cells xenografted onto nude mice and human tonsils. KBA.62 was selected because of its reactivity with melanocytic proliferations on both frozen and paraffin wax sections. Results-On immunoblotting, KBA.62 reacted with three bands of 140, 135 and 128 kD and two weak bands of 88 and 73 kD. In normal human tissues basal melanocytes in the epidermis did not react with this antibody and only occasional labelling of endothelial cells was noted. Of the human tumours, KBA.62 reacted strongly and uniformly with the majority of benign (21/21) and malignant (75/86) melanocytic proliferations. Staining was localised predominantly to the cell membrane with little or no cytoplasmic reactivity. Negative staining was observed in the majority of human non-melanocytic neoplasms, the exceptions being some carcinomas (11/89), particularly the well differentiated squamous cell type. This, however, was not thought to present a diagnostic problem. Conclusions-KBA.62 appears to be potentially useful in ascertaining the immunomorphological diagnosis of malignant melanoma in routinely processed paraffin wax sections.
引用
收藏
页码:826 / 831
页数:6
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